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Long-term monitoring of SARS-CoV-2 RNA in wastewater of the Frankfurt metropolitan area in Southern Germany

Agrawal, Shelesh ; Orschler, Laura ; Lackner, Susanne (2022)
Long-term monitoring of SARS-CoV-2 RNA in wastewater of the Frankfurt metropolitan area in Southern Germany.
In: Scientific Reports, 2022, 11
doi: 10.26083/tuprints-00021210
Article, Secondary publication, Publisher's Version

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Item Type: Article
Type of entry: Secondary publication
Title: Long-term monitoring of SARS-CoV-2 RNA in wastewater of the Frankfurt metropolitan area in Southern Germany
Language: English
Date: 10 May 2022
Place of Publication: Darmstadt
Year of primary publication: 2022
Publisher: Springer Nature
Journal or Publication Title: Scientific Reports
Volume of the journal: 11
Series Volume: 11
Collation: 7 Seiten
DOI: 10.26083/tuprints-00021210
Corresponding Links:
Origin: Secondary publication via sponsored Golden Open Access
Abstract:

Wastewater-based epidemiology (WBE) is a great approach that enables us to comprehensively monitor the community to determine the scale and dynamics of infections in a city, particularly in metropolitan cities with a high population density. Therefore, we monitored the time course of the SARS-CoV-2 RNA concentration in raw sewage in the Frankfurt metropolitan area, the European financial center. To determine the SARS-CoV-2 RNA concentration in sewage, we continuously collected 24 h composite samples twice a week from two wastewater treatment plant (WWTP) influents (Niederrad and Sindlingen) serving the Frankfurt metropolitan area and performed RT-qPCR analysis targeting three genes (N gene, S gene, and ORF1ab gene). In August, a resurgence in the SARS-CoV-2 RNA load was observed, reaching 3 × 10¹³ copies/day, which represented similar levels compared to April with approx. 2 × 10¹⁴ copies/day. This corresponds to a continuous increase again in COVID-19 cases in Frankfurt since August, with an average of 28.6 incidences, compared to 28.7 incidences in April. Different temporal dynamics were observed between different sampling points, indicating local dynamics in COVID-19 cases within the Frankfurt metropolitan area. The SARS-CoV-2 RNA load to the WWTP Niederrad ranged from approx. 4 × 10¹¹ to 1 × 10¹⁵ copies/day, the load to the WWTP Sindlingen from approx. 1 × 10¹¹ to 2 × 10¹⁴ copies/day, which resulted in a preceding increase in these loading in July ahead of the weekly averaged incidences. The study shows that WBE has the potential as an early warning system for SARS-CoV-2 infections and a monitoring system to identify global hotspots of COVID-19.

Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-212108
Classification DDC: 600 Technology, medicine, applied sciences > 600 Technology
Divisions: 13 Department of Civil and Environmental Engineering Sciences > Institute IWAR
Date Deposited: 10 May 2022 11:08
Last Modified: 14 Nov 2023 19:04
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/21210
PPN: 494614048
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