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The Influence of C-Ions and X-rays on Human Umbilical Vein Endothelial Cells

Helm, Alexander ; Lee, Ryonfa ; Durante, Marco ; Ritter, Sylvia (2024)
The Influence of C-Ions and X-rays on Human Umbilical Vein Endothelial Cells.
In: Frontiers in Oncology, 2016, 6
doi: 10.26083/tuprints-00016226
Article, Secondary publication, Publisher's Version

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Item Type: Article
Type of entry: Secondary publication
Title: The Influence of C-Ions and X-rays on Human Umbilical Vein Endothelial Cells
Language: English
Date: 8 March 2024
Place of Publication: Darmstadt
Year of primary publication: 20 January 2016
Place of primary publication: Lausanne
Publisher: Frontiers Media S.A.
Journal or Publication Title: Frontiers in Oncology
Volume of the journal: 6
Collation: 10 Seiten
DOI: 10.26083/tuprints-00016226
Corresponding Links:
Origin: Secondary publication DeepGreen
Abstract:

Damage to the endothelium of blood vessels, which may occur during radiotherapy, is discussed as a potential precursor to the development of cardiovascular disease. We thus chose human umbilical vein endothelial cells as a model system to examine the effect of low- and high-linear energy transfer (LET) radiation. Cells were exposed to 250 kV X-rays or carbon ions (C-ions) with the energies of either 9.8 MeV/u (LET = 170 keV/μm) or 91 MeV/u (LET = 28 keV/μm). Subculture of cells was performed regularly up to 46 days (~22 population doublings) post-irradiation. Immediately after exposure, cells were seeded for the colony forming assay. Additionally, at regular intervals, mitochondrial membrane potential (MMP) (JC-1 staining) and cellular senescence (senescence-associated β-galactosidase staining) were assessed. Cytogenetic damage was investigated by the micronucleus assay and the high-resolution multiplex fluorescence in situ hybridization (mFISH) technique. Analysis of radiation-induced damage shortly after exposure showed that C-ions are more effective than X-rays with respect to cell inactivation or the induction of cytogenetic damage (micronucleus assay) as observed in other cell systems. For 9.8 and 91 MeV/u C-ions, relative biological effectiveness values of 2.4 and 1.5 were obtained for cell inactivation. At the subsequent time points, the number of micronucleated cells decreased to the control level. Analysis of chromosomal damage by mFISH technique revealed aberrations frequently involving chromosome 13 irrespective of dose or radiation quality. Disruption of the MMP was seen only a few days after exposure to X-rays or C-ions. Cellular senescence was not altered by radiation at any time point investigated. Altogether, our data indicate that shortly after exposure C-ions were more effective in damaging endothelial cells than X-rays. However, late damage to endothelial cells was not found for the applied conditions and endpoints.

Uncontrolled Keywords: cardiovascular disease, endothelial cells, high-LET radiation, carbon ions, carbon ion therapy, chromosome 13, micronucleus formation, senescence-associated β-galactosidase
Identification Number: Artikel-ID: 5
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-162260
Additional Information:

This article is part of the Research Topic: Charged Particles in Oncology

Specialty section: This article was submitted to Radiation Oncology, a section of the journal Frontiers in Oncology

Classification DDC: 500 Science and mathematics > 530 Physics
600 Technology, medicine, applied sciences > 610 Medicine and health
Divisions: 05 Department of Physics > Institute for Condensed Matter Physics
Date Deposited: 08 Mar 2024 13:07
Last Modified: 30 Apr 2024 14:28
SWORD Depositor: Deep Green
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/16226
PPN: 51768246X
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