Quartieri, Martina ; Puspitasari, Anggraeini ; Vitacchio, Tamara ; Durante, Marco ; Tinganelli, Walter (2024)
The role of hypoxia and radiation in developing a CTCs-like phenotype in murine osteosarcoma cells.
In: Frontiers in Cell and Developmental Biology, 2023, 11
doi: 10.26083/tuprints-00027154
Article, Secondary publication, Publisher's Version
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Item Type: | Article |
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Type of entry: | Secondary publication |
Title: | The role of hypoxia and radiation in developing a CTCs-like phenotype in murine osteosarcoma cells |
Language: | English |
Date: | 4 June 2024 |
Place of Publication: | Darmstadt |
Year of primary publication: | 16 November 2023 |
Place of primary publication: | Lausanne |
Publisher: | Frontiers Media S.A. |
Journal or Publication Title: | Frontiers in Cell and Developmental Biology |
Volume of the journal: | 11 |
Collation: | 13 Seiten |
DOI: | 10.26083/tuprints-00027154 |
Corresponding Links: | |
Origin: | Secondary publication DeepGreen |
Abstract: | Introduction: Cancer treatment has evolved significantly, yet concerns about tumor recurrence and metastasis persist. Within the dynamic tumor microenvironment, a subpopulation of mesenchymal tumor cells, known as Circulating Cancer Stem Cells (CCSCs), express markers like CD133, TrkB, and CD47, making them radioresistant and pivotal to metastasis. Hypoxia intensifies their stemness, complicating their identification in the bloodstream. This study investigates the interplay of acute and chronic hypoxia and radiation exposure in selecting and characterizing cells with a CCSC-like phenotype. Methods: LM8 murine osteosarcoma cells were cultured and subjected to normoxic (21% O2) and hypoxic (1% O2) conditions. We employed Sphere Formation and Migration Assays, Western Blot analysis, CD133 Cell Sorting, and CD133+ Fluorescent Activated Cell Sorting (FACS) analysis with a focus on TrkB antibody to assess the effects of acute and chronic hypoxia, along with radiation exposure. Results: Our findings demonstrate that the combination of radiation and acute hypoxia enhances stemness, while chronic hypoxia imparts a cancer stem-like phenotype in murine osteosarcoma cells, marked by increased migration and upregulation of CCSC markers, particularly TrkB and CD47. These insights offer a comprehensive understanding of the interactions between radiation, hypoxia, and cellular responses in the context of cancer treatment. Discussion: This study elucidates the complex interplay among radiation, hypoxia, and cellular responses, offering valuable insights into the intricacies and potential advancements in cancer treatment. |
Uncontrolled Keywords: | tumor metastasis, cancer stem cells, circulating tumor cells, tumor hypoxia, radiation |
Identification Number: | Artikel-ID: 1222809 |
Status: | Publisher's Version |
URN: | urn:nbn:de:tuda-tuprints-271544 |
Additional Information: | This article is part of the Research Topic: Mechanisms of Metastasis from Circulating and Disseminated Tumor Cells Sec. Cancer Cell Biology |
Classification DDC: | 500 Science and mathematics > 530 Physics 500 Science and mathematics > 570 Life sciences, biology |
Divisions: | 05 Department of Physics > Institute for Condensed Matter Physics |
Date Deposited: | 04 Jun 2024 12:51 |
Last Modified: | 07 Jun 2024 07:23 |
SWORD Depositor: | Deep Green |
URI: | https://tuprints.ulb.tu-darmstadt.de/id/eprint/27154 |
PPN: | 518861341 |
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