2025

Schoenfeld, Katrin ; Harwardt, Julia ; Kolmar, Harald (2025)
Better safe than sorry: dual targeting antibodies for cancer immunotherapy.
In: Biological Chemistry, 2024
doi: 10.26083/tuprints-00027608
Article, Secondary publication, Publisher's Version

2024

Bogen, Jan P. ; Hinz, Steffen C. ; Grzeschik, Julius ; Ebenig, Aileen ; Krah, Simon ; Zielonka, Stefan ; Kolmar, Harald (2024)
Dual Function pH Responsive Bispecific Antibodies for Tumor Targeting and Antigen Depletion in Plasma.
In: Frontiers in Immunology, 2019, 10
doi: 10.26083/tuprints-00015735
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Hillscher, Laura M. ; Liebich, Valentina J. ; Avrutina, Olga ; Biesalski, Markus ; Kolmar, Harald (2024)
Functional paper-based materials for diagnostics.
In: ChemTexts, 2021, 7 (2)
doi: 10.26083/tuprints-00023549
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Pfeifer Serrahima, Jordi ; Schoenfeld, Katrin ; Kühnel, Ines ; Harwardt, Julia ; Macarrón Palacios, Arturo ; Prüfer, Maren ; Kolaric, Margareta ; Oberoi, Pranav ; Kolmar, Harald ; Wels, Winfried S. (2024)
Bispecific killer cell engagers employing species cross-reactive NKG2D binders redirect human and murine lymphocytes to ErbB2/HER2-positive malignancies.
In: Frontiers in Immunology, 2024, 15
doi: 10.26083/tuprints-00028083
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Grotz, Melanie ; Gijzel, Lieke van ; Bitsch, Peter ; Carrara, Stefania C. ; Kolmar, Harald ; Garg, Sakshi (2024)
Mimicking the immunosuppressive impact of fibroblasts in a 3D multicellular spheroid model.
In: Frontiers in Drug Discovery, 2024, 4
doi: 10.26083/tuprints-00027873
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Reinwarth, Michael ; Avrutina, Olga ; Fabritz, Sebastian ; Kolmar, Harald (2024)
Fragmentation follows structure: top-down mass spectrometry elucidates the topology of engineered cystine-knot miniproteins.
In: PLOS ONE, 2014, 9 (10)
doi: 10.26083/tuprints-00027627
Article, Secondary publication, Publisher's Version

Oliviero, Claudia ; Hinz, Steffen C. ; Bogen, Jan P. ; Kornmann, Henri ; Hock, Björn ; Kolmar, Harald ; Hagens, Gerrit (2024)
Generation of a host cell line containing a MAR-rich landing pad for site-specific integration and expression of transgenes.
In: Biotechnology Progress, 2022, 38 (4)
doi: 10.26083/tuprints-00027669
Article, Secondary publication, Publisher's Version

Djemal, Leila ; Fournier, Clemence ; Hagen, Joerg von ; Kolmar, Harald ; Deparis, Véronique (2024)
Review: High temperature short time treatment of cell culture media and feed solutions to mitigate adventitious viral contamination in the biopharmaceutical industry.
In: Biotechnology Progress, 2021, 37 (3)
doi: 10.26083/tuprints-00027676
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Hadjabdelhafid-Parisien, Adem ; Bitsch, Sebastian ; Macarrón Palacios, Arturo ; Deweid, Lukas ; Kolmar, Harald ; Pelletier, Joelle N. (2024)
Tag-free, specific conjugation of glycosylated IgG1 antibodies using microbial transglutaminase.
In: RSC Advances, 2022, 12 (52)
doi: 10.26083/tuprints-00027672
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Hofmann, Tim ; Schmidt, Johannes ; Ciesielski, Elke ; Becker, Stefan ; Rysiok, Thomas ; Schütte, Mark ; Toleikis, Lars ; Kolmar, Harald ; Doerner, Achim (2024)
Intein mediated high throughput screening for bispecific antibodies.
In: mAbs, 2020, 12 (1)
doi: 10.26083/tuprints-00027688
Article, Secondary publication, Publisher's Version

Rhiel, Laura ; Krah, Simon ; Günther, Ralf ; Becker, Stefan ; Kolmar, Harald ; Hock, Björn (2024)
REAL-Select: Full-Length Antibody Display and Library Screening by Surface Capture on Yeast Cells.
In: PLOS ONE, 2014, 9 (12)
doi: 10.26083/tuprints-00027624
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Schröter, Christian ; Becker, Jan ; Krah, Simon ; Zielonka, Stefan ; Doerner, Achim ; Rhiel, Laura ; Günther, Ralf ; Toleikis, Lars ; Kolmar, Harald ; Hock, Björn ; Becker, Stefan (2024)
Selection of Antibodies with Tailored Properties by Application of High-Throughput Multiparameter Fluorescence-Activated Cell Sorting of Yeast-Displayed Immune Libraries.
In: Molecular Biotechnology, 2018, 60
doi: 10.26083/tuprints-00027671
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Carrara, Stefania C. ; Bogen, Jan P. ; Fiebig, David ; Grzeschik, Julius ; Hock, Björn ; Kolmar, Harald (2024)
Targeted Phagocytosis Induction for Cancer Immunotherapy via Bispecific MerTK-Engaging Antibodies.
In: International Journal of Molecular Sciences, 2022, 23 (24)
doi: 10.26083/tuprints-00027673
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Arras, Paul ; Zimmermann, Jasmin ; Lipinski, Britta ; Valldorf, Bernhard ; Evers, Andreas ; Elter, Desislava ; Krah, Simon ; Doerner, Achim ; Guarnera, Enrico ; Siegmund, Vanessa ; Kolmar, Harald ; Pekar, Lukas ; Zielonka, Stefan (2024)
Bovine ultralong CDR-H3 derived knob paratopes elicit potent TNF-α neutralization and enable the generation of novel adalimumab-based antibody architectures with augmented features.
In: Biological Chemistry, 2024
doi: 10.26083/tuprints-00027605
Article, Secondary publication, Publisher's Version

Zhou, Xueyuan ; Geyer, Felix Klaus ; Happel, Dominic ; Takimoto, Jeffrey ; Kolmar, Harald ; Rabinovich, Brian (2024)
Using protein geometry to optimize cytotoxicity and the cytokine window of a ROR1 specific T cell engager.
In: Frontiers in Immunology, 2024, 15
doi: 10.26083/tuprints-00027164
Article, Secondary publication, Publisher's Version

Uth, Christina ; Englert, Simon ; Avrutina, Olga ; Kolmar, Harald ; Knauer, Sascha (2024)
Novel amino‐Li resin for water‐based solid‐phase peptide synthesis.
In: Journal of Peptide Science, 2023, 29 (12)
doi: 10.26083/tuprints-00027214
Article, Secondary publication, Publisher's Version

Ulitzka, Michael ; Harwardt, Julia ; Lipinski, Britta ; Tran, Hue ; Hock, Björn ; Kolmar, Harald (2024)
Potent Apoptosis Induction by a Novel Trispecific B7-H3xCD16xTIGIT 2+1 Common Light Chain Natural Killer Cell Engager.
In: Molecules, 2024, 29 (5)
doi: 10.26083/tuprints-00027124
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Elter, Adrian ; Bogen, Jan P. ; Habermann, Jan ; Kolmar, Harald (2024)
Vom Huhn abgeleitete Antikörper für Diagnostik und Immuntherapie.
In: BIOspektrum : Das Magazin für Biowissenschaften, 2021, 27 (5)
doi: 10.26083/tuprints-00023545
Article, Secondary publication, Publisher's Version

Jäger, Sebastian ; Dickgiesser, Stephan ; Tonillo, Jason ; Hecht, Stefan ; Kolmar, Harald ; Schröter, Christian (2024)
EGFR binding Fc domain-drug conjugates: stable and highly potent cytotoxic molecules mediate selective cell killing.
In: Biological Chemistry, 2022, 403 (5-6)
doi: 10.26083/tuprints-00027284
Article, Secondary publication, Publisher's Version

Macarrón Palacios, Arturo ; Grzeschik, Julius ; Deweid, Lukas ; Krah, Simon ; Zielonka, Stefan ; Rösner, Thies ; Peipp, Matthias ; Valerius, Thomas ; Kolmar, Harald (2024)
Specific Targeting of Lymphoma Cells Using Semisynthetic Anti-Idiotype Shark Antibodies.
In: Frontiers in Immunology, 2020, 11
doi: 10.26083/tuprints-00017467
Article, Secondary publication, Publisher's Version

Böhm, Alexander ; Trosien, Simon ; Avrutina, Olga ; Kolmar, Harald ; Biesalski, Markus (2024)
Covalent Attachment of Enzymes to Paper Fibers for Paper-Based Analytical Devices.
In: Frontiers in Chemistry, 2018, 6
doi: 10.26083/tuprints-00015860
Article, Secondary publication, Publisher's Version

Elter, Adrian ; Bogen, Jan P. ; Hinz, Steffen C. ; Fiebig, David ; Macarrón Palacios, Arturo ; Grzeschik, Julius ; Hock, Björn ; Kolmar, Harald (2024)
Humanization of Chicken‐Derived scFv Using Yeast Surface Display and NGS Data Mining.
In: Biotechnology Journal : Systems & Synthetic Biology, Nanobiotech, Medicine, 2021, 16 (3)
doi: 10.26083/tuprints-00017792
Article, Secondary publication, Publisher's Version

Becker, Bastian ; Englert, Simon ; Schneider, Hendrik ; Yanakieva, Desislava ; Hofmann, Sarah ; Dombrowsky, Carolin ; Macarrón Palacios, Arturo ; Bitsch, Sebastian ; Elter, Adrian ; Meckel, Tobias ; Kugler, Benedikt ; Schirmacher, Anastasyia ; Avrutina, Olga ; Diederichsen, Ulf ; Kolmar, Harald (2024)
Multivalent dextran hybrids for efficient cytosolic delivery of biomolecular cargoes.
In: Journal of Peptide Science : the official Journal of the European Peptide Society, 2021, 27 (4)
doi: 10.26083/tuprints-00017809
Article, Secondary publication, Publisher's Version

Bogen, Jan P. ; Storka, Juliana ; Yanakieva, Desislava ; Fiebig, David ; Grzeschik, Julius ; Hock, Björn ; Kolmar, Harald (2024)
Isolation of Common Light Chain Antibodies from Immunized Chickens Using Yeast Biopanning and Fluorescence‐Activated Cell Sorting.
In: Biotechnology Journal : Systems and Synthetic Biology, Nanobiotech, Medicine, 2020, 16 (3)
doi: 10.26083/tuprints-00017449
Article, Secondary publication, Publisher's Version

Djemal, Leïla ; Hagen, Joerg von ; Kolmar, Harald ; Deparis, Véronique (2024)
Characterization of soy protein hydrolysates and influence of its iron content on monoclonal antibody production by a murine hybridoma cell line.
In: Biotechnology Progress, 2021, 37 (4)
doi: 10.26083/tuprints-00021528
Article, Secondary publication, Publisher's Version

Pekar, Lukas ; Klewinghaus, Daniel ; Arras, Paul ; Carrara, Stefania C. ; Harwardt, Julia ; Krah, Simon ; Yanakieva, Desislava ; Toleikis, Lars ; Smider, Vaughn V. ; Kolmar, Harald ; Zielonka, Stefan (2024)
Milking the Cow: Cattle-Derived Chimeric Ultralong CDR-H3 Antibodies and Their Engineered CDR-H3-Only Knobbody Counterparts Targeting Epidermal Growth Factor Receptor Elicit Potent NK Cell-Mediated Cytotoxicity.
In: Frontiers in Immunology, 2021, 12
doi: 10.26083/tuprints-00019967
Article, Secondary publication, Publisher's Version

Krah, Simon ; Kolmar, Harald ; Becker, Stefan ; Zielonka, Stefan (2024)
Engineering IgG-Like Bispecific Antibodies — An Overview.
In: Antibodies, 2018, 7 (3)
doi: 10.26083/tuprints-00015758
Article, Secondary publication, Publisher's Version

Bitsch, Peter ; Baum, Eva S. ; Beltrán Hernández, Irati ; Bitsch, Sebastian Harald ; Harwood, Jakob ; Oliveira, Sabrina ; Kolmar, Harald (2024)
Penetration of Nanobody-Dextran Polymer Conjugates through Tumor Spheroids.
In: Pharmaceutics, 2023, 15 (10)
doi: 10.26083/tuprints-00024648
Article, Secondary publication, Publisher's Version

Carrara, Stefania C. ; Fiebig, David ; Bogen, Jan P. ; Grzeschik, Julius ; Hock, Björn ; Kolmar, Harald (2024)
Recombinant Antibody Production Using a Dual-Promoter Single Plasmid System.
In: Antibodies, 2021, 10 (2)
doi: 10.26083/tuprints-00019564
Article, Secondary publication, Publisher's Version

2023

Bogen, Jan Patrick ; Carrara, Stefania Candela ; Fiebig, David ; Grzeschik, Julius ; Hock, Björn ; Kolmar, Harald (2023)
Supplemental Information with antibody sequences.
doi: 10.26083/tuprints-00017895
Data, Primary publication, Publisher's Version

Harwardt, Julia ; Carrara, Stefania C. ; Bogen, Jan P. ; Schoenfeld, Katrin ; Grzeschik, Julius ; Hock, Björn ; Kolmar, Harald (2023)
Generation of a symmetrical trispecific NK cell engager based on a two-in-one antibody.
In: Frontiers in Immunology, 2023, 14
doi: 10.26083/tuprints-00023673
Article, Secondary publication, Publisher's Version

2022

Ali, Ataurehman ; Happel, Dominic ; Habermann, Jan ; Schoenfeld, Katrin ; Macarrón Palacios, Arturo ; Bitsch, Sebastian ; Englert, Simon ; Schneider, Hendrik ; Avrutina, Olga ; Fabritz, Sebastian ; Kolmar, Harald (2022)
Sactipeptide Engineering by Probing the Substrate Tolerance of a Thioether‐Bond‐Forming Sactisynthase.
In: Angewandte Chemie International Edition, 2022, 61 (45)
doi: 10.26083/tuprints-00022904
Article, Secondary publication, Publisher's Version

Ali, Ataurehman ; Happel, Dominic ; Habermann, Jan ; Schoenfeld, Katrin ; Macarrón Palacios, Arturo ; Bitsch, Sebastian ; Englert, Simon ; Schneider, Hendrik ; Avrutina, Olga ; Fabritz, Sebastian ; Kolmar, Harald (2022)
Untersuchungen zur Substrattoleranz von Sactisynthasen für die Generierung Thioether‐verbrückter Sactipeptide mit neuen Eigenschaften.
In: Angewandte Chemie, 2022, 134 (45)
doi: 10.26083/tuprints-00022902
Article, Secondary publication, Publisher's Version

Lerma Romero, Jorge A. ; Meyners, Christian ; Christmann, Andreas ; Reinbold, Lisa M. ; Charalampidou, Anna ; Hausch, Felix ; Kolmar, Harald (2022)
Binding pocket stabilization by high-throughput screening of yeast display libraries.
In: Frontiers in Molecular Biosciences, 2022, 9
doi: 10.26083/tuprints-00022911
Article, Secondary publication, Publisher's Version

Carrara, Stefania C. ; Harwardt, Julia ; Grzeschik, Julius ; Hock, Björn ; Kolmar, Harald (2022)
TriTECM: A tetrafunctional T-cell engaging antibody with built-in risk mitigation of cytokine release syndrome.
In: Frontiers in Immunology, 2022, 13
doi: 10.26083/tuprints-00022942
Article, Secondary publication, Publisher's Version

Darvin, Maxim E. ; Lademann, Jürgen ; Hagen, Jörg von ; Lohan, Silke B. ; Kolmar, Harald ; Meinke, Martina C. ; Jung, Sora (2022)
Carotenoids in Human Skin In Vivo: Antioxidant and Photo-Protectant Role against External and Internal Stressors.
In: Antioxidants, 2022, 11 (8)
doi: 10.26083/tuprints-00021856
Article, Secondary publication, Publisher's Version

Fiebig, David ; Bogen, Jan P. ; Carrara, Stefania C. ; Deweid, Lukas ; Zielonka, Stefan ; Grzeschik, Julius ; Hock, Björn ; Kolmar, Harald (2022)
Streamlining the Transition From Yeast Surface Display of Antibody Fragment Immune Libraries to the Production as IgG Format in Mammalian Cells.
In: Frontiers in Bioengineering and Biotechnology, 2022, 10
doi: 10.26083/tuprints-00021336
Article, Secondary publication, Publisher's Version

Klewinghaus, Daniel ; Pekar, Lukas ; Arras, Paul ; Krah, Simon ; Valldorf, Bernhard ; Kolmar, Harald ; Zielonka, Stefan (2022)
Grabbing the Bull by Both Horns: Bovine Ultralong CDR-H3 Paratopes Enable Engineering of ‘Almost Natural’ Common Light Chain Bispecific Antibodies Suitable For Effector Cell Redirection.
In: Frontiers in Immunology, 2022, 12
doi: 10.26083/tuprints-00020369
Article, Secondary publication, Publisher's Version

Harwardt, Julia ; Bogen, Jan P. ; Carrara, Stefania C. ; Ulitzka, Michael ; Grzeschik, Julius ; Hock, Björn ; Kolmar, Harald (2022)
A Generic Strategy to Generate Bifunctional Two-in-One Antibodies by Chicken Immunization.
In: Frontiers in Immunology, 2022, 13
doi: 10.26083/tuprints-00021242
Article, Secondary publication, Publisher's Version

Elter, Adrian Elias Curd ; Bock, Tina ; Spiehl, Dieter ; Russo, Giulio ; Hinz, Steffen C. ; Bitsch, Sebastian ; Baum, Eva ; Langhans, Markus ; Meckel, Tobias ; Dörsam, Edgar ; Kolmar, Harald ; Schwall, Gerhard (2022)
Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays.
In: Scientific Reports, 2022, 11
doi: 10.26083/tuprints-00021201
Article, Secondary publication, Publisher's Version

Oloketuyi, Sandra ; Bernedo, Robert ; Christmann, Andreas ; Borkowska, Justyna ; Cazzaniga, Giulia ; Schuchmann, Horst Wilhelm ; Niedziółka-Jönsson, Joanna ; Szot-Karpińska, Katarzyna ; Kolmar, Harald ; Marco, Ario de (2022)
Native llama Nanobody Library Panning Performed by Phage and Yeast Display Provides Binders Suitable for C-Reactive Protein Detection.
In: Biosensors, 2022, 11 (12)
doi: 10.26083/tuprints-00020274
Article, Secondary publication, Publisher's Version

2021

Elter, Adrian ; Yanakieva, Desislava ; Fiebig, David ; Hallstein, Kerstin ; Becker, Stefan ; Betz, Ulrich ; Kolmar, Harald (2021)
Protease-Activation of Fc-Masked Therapeutic Antibodies to Alleviate Off-Tumor Cytotoxicity.
In: Frontiers in Immunology, 2021, 12
doi: 10.26083/tuprints-00019456
Article, Secondary publication, Publisher's Version

Bogen, Jan Patrick ; Grzeschik, Julius ; Jakobsen, Jørn ; Bähre, Alexandra ; Hock, Björn ; Kolmar, Harald (2021)
Treating Bladder Cancer: Engineering of Current and Next Generation Antibody-, Fusion Protein-, mRNA-, Cell- and Viral-Based Therapeutics.
In: Frontiers in Oncology, 2021, 11
doi: 10.26083/tuprints-00019435
Article, Secondary publication, Publisher's Version

Schneider, Hendrik ; Englert, Simon ; Macarrón Palacios, Arturo ; Lerma Romero, Jorge Alberto ; Ali, Ataurehman ; Avrutina, Olga ; Kolmar, Harald (2021)
Synthetic Integrin-Targeting Dextran-Fc Hybrids Efficiently Inhibit Tumor Proliferation In Vitro.
In: Frontiers in Chemistry, 2021, 9
doi: 10.26083/tuprints-00019401
Article, Secondary publication, Publisher's Version

Bogen, Jan P. ; Carrara, Stefania C. ; Fiebig, David ; Grzeschik, Julius ; Hock, Björn ; Kolmar, Harald (2021)
Design of a Trispecific Checkpoint Inhibitor and Natural Killer Cell Engager Based on a 2 + 1 Common Light Chain Antibody Architecture.
In: Frontiers in Immunology, 2021, 12
doi: 10.26083/tuprints-00019364
Article, Secondary publication, Publisher's Version

Bogen, Jan P. ; Carrara, Stefania C. ; Fiebig, David ; Grzeschik, Julius ; Hock, Björn ; Kolmar, Harald (2021)
Expeditious Generation of Biparatopic Common Light Chain Antibodies via Chicken Immunization and Yeast Display Screening.
In: Frontiers in Immunology, 2020, 11
doi: 10.26083/tuprints-00019264
Article, Secondary publication, Publisher's Version

Hinz, Steffen C. ; Elter, Adrian ; Rammo, Oliver ; Schwämmle, Achim ; Ali, Ataurehman ; Zielonka, Stefan ; Herget, Thomas ; Kolmar, Harald (2021)
A Generic Procedure for the Isolation of pH- and Magnesium-Responsive Chicken scFvs for Downstream Purification of Human Antibodies.
In: Frontiers in Bioengineering and Biotechnology, 2020, 8
doi: 10.26083/tuprints-00019238
Article, Secondary publication, Publisher's Version

Yanakieva, Desislava ; Elter, Adrian ; Bratsch, Jens ; Friedrich, Karlheinz ; Becker, Stefan ; Kolmar, Harald (2021)
FACS-Based Functional Protein Screening via Microfluidic Co-encapsulation of Yeast Secretor and Mammalian Reporter Cells.
In: Scientific Reports, 2020, (10)
doi: 10.26083/tuprints-00018649
Article, Secondary publication, Publisher's Version

2019

Hofmann, Anja ; Falk, Johannes ; Prangemeier, Tim ; Happel, Dominic ; Köber, Adrian ; Christmann, Andreas ; Koeppl, Heinz ; Kolmar, Harald (2019)
A tightly regulated and adjustable CRISPR-dCas9 based AND gate in yeast.
In: Nucleic Acids Research, 2018, 47 (1)
Article, Secondary publication, Publisher's Version

2017

Könning, Doreen ; Rhiel, Laura ; Empting, Martin ; Grzeschik, Julius ; Sellmann, Carolin ; Schröter, Christian ; Zielonka, Stefan ; Dickgießer, Stephan ; Pirzer, Thomas ; Yanakieva, Desislava ; Becker, Stefan ; Kolmar, Harald (2017)
Semi-synthetic vNAR libraries screened against therapeutic antibodies primarily deliver anti-idiotypic binders.
In: Scientific Reports, 2017, (1)
Article, Secondary publication, Publisher's Version