TU Darmstadt / ULB / TUprints

FACS-Based Functional Protein Screening via Microfluidic Co-encapsulation of Yeast Secretor and Mammalian Reporter Cells

Yanakieva, Desislava ; Elter, Adrian ; Bratsch, Jens ; Friedrich, Karlheinz ; Becker, Stefan ; Kolmar, Harald (2021):
FACS-Based Functional Protein Screening via Microfluidic Co-encapsulation of Yeast Secretor and Mammalian Reporter Cells. (Publisher's Version)
In: Scientific Reports, (10), Springer Nature, e-ISSN 2045-2322,
DOI: 10.26083/tuprints-00018649,
[Article]

[img]
Preview
Text
s41598-020-66927-5.pdf
Available under CC BY 4.0 International - Creative Commons, Attribution.

Download (4MB) | Preview
Item Type: Article
Origin: Secondary publication via sponsored Golden Open Access
Status: Publisher's Version
Title: FACS-Based Functional Protein Screening via Microfluidic Co-encapsulation of Yeast Secretor and Mammalian Reporter Cells
Language: English
Abstract:

In this study, we present a straightforward approach for functional cell-based screening by co-encapsulation of secretor yeast cells and reporter mammalian cells in millions of individual agarose-containing microdroplets. Our system is compatible with ultra-high-throughput selection utilizing standard fluorescence-activated cell sorters (FACS) without need of extensive adaptation and optimization. In a model study we co-encapsulated murine interleukin 3 (mIL-3)-secreting S. cerevisiae cells with murine Ba/F3 reporter cells, which express green fluorescent protein (GFP) upon stimulation with mIL-3, and could observe specific and robust induction of fluorescence signal compared to a control with yeast cells secreting a non-functional mIL-3 mutant. We demonstrate the successful enrichment of activating mIL-3 wt-secreting yeast cells from a 1:10,000 dilution in cells expressing the inactive cytokine variant by two consecutive cycles of co-encapsulation and FACS. This indicates the suitability of the presented strategy for functional screening of high-diversity yeast-based libraries and demonstrates its potential for the efficient isolation of clones secreting bioactive recombinant proteins.

Journal or Publication Title: Scientific Reports
Number: 10
Publisher: Springer Nature
Collation: 13 Seiten
Classification DDC: 500 Naturwissenschaften und Mathematik > 540 Chemie
Divisions: 07 Department of Chemistry > Fachgebiet Biochemie
Date Deposited: 20 Jul 2021 08:45
Last Modified: 20 Jul 2021 08:46
DOI: 10.26083/tuprints-00018649
Corresponding Links:
URN: urn:nbn:de:tuda-tuprints-186496
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/18649
Export:
Actions (login required)
View Item View Item