Entwicklung Fluoreszenz-basierter Assays zur kinetischen Untersuchung neuer Enzymvarianten

Scheidt, Thomas (2017)
Entwicklung Fluoreszenz-basierter Assays zur kinetischen Untersuchung neuer Enzymvarianten.
Technische Universität Darmstadt
Ph.D. Thesis, Primary publication

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Item Type:

Ph.D. Thesis

Type of entry:

Primary publication

Title:

Entwicklung Fluoreszenz-basierter Assays zur kinetischen Untersuchung neuer Enzymvarianten

Language:

German

Referees:

Fessner, Prof. Dr. Wolf-Dieter ; Kolmar, Prof. Dr. Harald

Date:

8 March 2017

Place of Publication:

Darmstadt

Date of oral examination:

6 March 2017

Abstract:

Entwicklung verschiedener photometrischer und Fluoreszenz-basierter Assays zur untersuchung künstlich erzeugter Varianten der Fruktose-6-phosphat Aldolase, sowie Synthese der dafür benötigten Substrate und Erzeugung dere Enzym-Varianten mittels Punktmutation.

Entwicklung einer modularen Synthese für strukturverwandte enantiomeren reiner Aminsubstrate und Entwicklung des ersten Fluoreszenz-Basierten Assays zu kinetischen Untersuchung von w-Transaminasen

Alternative Abstract:

Alternative Abstract

Language

Development of several photometric and fluorescence-based assays for the screening of artificial variants of the Fructose-6-phosphate aldolase towards non-natural reactions. The project included the establishment of a modular synthesis for the substrates as well as planning point mutations to improve the enzyme’s activity and developing new assay concepts.

Development of a modular synthesis of structurally related enantio-pure chirale amines from corresponding alcohols created by Grignard-addition. The amines, bearing a methoxinaphthyl-based fluorophore, were later to be used as assay substrates for screening of w-transaminases.

English

URN:

urn:nbn:de:tuda-tuprints-60811

Classification DDC:

500 Science and mathematics > 540 Chemistry

Divisions:

07 Department of Chemistry > Clemens-Schöpf-Institut > Organ Chemistry

Date Deposited:

08 Mar 2017 12:34

Last Modified:

09 Jul 2020 01:34

URI: