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An Outer Membrane‐Inspired Polymer Coating Protects and Endows Escherichia coli with Novel Functionalities

Belluati, Andrea ; Harley, Iain ; Lieberwirth, Ingo ; Bruns, Nico (2024)
An Outer Membrane‐Inspired Polymer Coating Protects and Endows Escherichia coli with Novel Functionalities.
In: Small : nano micro, 2023, 19 (46)
doi: 10.26083/tuprints-00027177
Article, Secondary publication, Publisher's Version

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Item Type: Article
Type of entry: Secondary publication
Title: An Outer Membrane‐Inspired Polymer Coating Protects and Endows Escherichia coli with Novel Functionalities
Language: English
Date: 4 June 2024
Place of Publication: Darmstadt
Year of primary publication: 15 November 2023
Place of primary publication: Weinheim
Publisher: Wiley-VCH
Journal or Publication Title: Small : nano micro
Volume of the journal: 19
Issue Number: 46
Collation: 8 Seiten
DOI: 10.26083/tuprints-00027177
Corresponding Links:
Origin: Secondary publication DeepGreen
Abstract:

A bio‐inspired membrane made of Pluronic L‐121 is produced around Escherichia coli thanks to the simple co‐extrusion of bacteria and polymer vesicles. The block copolymer‐coated bacteria can withstand various harsh shocks, for example, temperature, pressure, osmolarity, and chemical agents. The polymer membrane also makes the bacteria resistant to enzymatic digestion and enables them to degrade toxic compounds, improving their performance as whole‐cell biocatalysts. Moreover, the polymer membrane acts as an anchor layer for the surface modification of the bacteria. Being decorated with α‐amylase or lysozyme, the cells are endowed with the ability to digest starch or self‐predatory bacteria are created. Thus, without any genetic engineering, the phenotype of encapsulated bacteria is changed as they become sturdier and gain novel metabolic functionalities.

Uncontrolled Keywords: block copolymers, cytoprotection, membrane functionalization, membranes, single‐cell encapsulation
Identification Number: Artikel-ID: 2303384
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-271772
Classification DDC: 500 Science and mathematics > 540 Chemistry
500 Science and mathematics > 570 Life sciences, biology
Divisions: Interdisziplinäre Forschungsprojekte > Centre for Synthetic Biology
07 Department of Chemistry > Ernst-Berl-Institut > Fachgebiet Makromolekulare Chemie
Date Deposited: 04 Jun 2024 12:35
Last Modified: 19 Sep 2024 13:41
SWORD Depositor: Deep Green
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/27177
PPN: 518866424
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