Belluati, Andrea ; Harley, Iain ; Lieberwirth, Ingo ; Bruns, Nico (2024)
An Outer Membrane‐Inspired Polymer Coating Protects and Endows Escherichia coli with Novel Functionalities.
In: Small : nano micro, 2023, 19 (46)
doi: 10.26083/tuprints-00027177
Article, Secondary publication, Publisher's Version
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Item Type: | Article |
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Type of entry: | Secondary publication |
Title: | An Outer Membrane‐Inspired Polymer Coating Protects and Endows Escherichia coli with Novel Functionalities |
Language: | English |
Date: | 4 June 2024 |
Place of Publication: | Darmstadt |
Year of primary publication: | 15 November 2023 |
Place of primary publication: | Weinheim |
Publisher: | Wiley-VCH |
Journal or Publication Title: | Small : nano micro |
Volume of the journal: | 19 |
Issue Number: | 46 |
Collation: | 8 Seiten |
DOI: | 10.26083/tuprints-00027177 |
Corresponding Links: | |
Origin: | Secondary publication DeepGreen |
Abstract: | A bio‐inspired membrane made of Pluronic L‐121 is produced around Escherichia coli thanks to the simple co‐extrusion of bacteria and polymer vesicles. The block copolymer‐coated bacteria can withstand various harsh shocks, for example, temperature, pressure, osmolarity, and chemical agents. The polymer membrane also makes the bacteria resistant to enzymatic digestion and enables them to degrade toxic compounds, improving their performance as whole‐cell biocatalysts. Moreover, the polymer membrane acts as an anchor layer for the surface modification of the bacteria. Being decorated with α‐amylase or lysozyme, the cells are endowed with the ability to digest starch or self‐predatory bacteria are created. Thus, without any genetic engineering, the phenotype of encapsulated bacteria is changed as they become sturdier and gain novel metabolic functionalities. |
Uncontrolled Keywords: | block copolymers, cytoprotection, membrane functionalization, membranes, single‐cell encapsulation |
Identification Number: | Artikel-ID: 2303384 |
Status: | Publisher's Version |
URN: | urn:nbn:de:tuda-tuprints-271772 |
Classification DDC: | 500 Science and mathematics > 540 Chemistry 500 Science and mathematics > 570 Life sciences, biology |
Divisions: | Interdisziplinäre Forschungsprojekte > Centre for Synthetic Biology 07 Department of Chemistry > Ernst-Berl-Institut > Fachgebiet Makromolekulare Chemie |
Date Deposited: | 04 Jun 2024 12:35 |
Last Modified: | 19 Sep 2024 13:41 |
SWORD Depositor: | Deep Green |
URI: | https://tuprints.ulb.tu-darmstadt.de/id/eprint/27177 |
PPN: | 518866424 |
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