TU Darmstadt / ULB / TUprints

Streamlining the Transition From Yeast Surface Display of Antibody Fragment Immune Libraries to the Production as IgG Format in Mammalian Cells

Fiebig, David ; Bogen, Jan P. ; Carrara, Stefania C. ; Deweid, Lukas ; Zielonka, Stefan ; Grzeschik, Julius ; Hock, Björn ; Kolmar, Harald (2022)
Streamlining the Transition From Yeast Surface Display of Antibody Fragment Immune Libraries to the Production as IgG Format in Mammalian Cells.
In: Frontiers in Bioengineering and Biotechnology, 2022, 10
doi: 10.26083/tuprints-00021336
Article, Secondary publication, Publisher's Version

[img] Text
fbioe-10-794389.pdf
Copyright Information: CC BY 4.0 International - Creative Commons, Attribution.

Download (2MB)
[img] Text
21336-Suppl.PDF
Copyright Information: CC BY 4.0 International - Creative Commons, Attribution.

Download (1MB)
Item Type: Article
Type of entry: Secondary publication
Title: Streamlining the Transition From Yeast Surface Display of Antibody Fragment Immune Libraries to the Production as IgG Format in Mammalian Cells
Language: English
Date: 20 May 2022
Place of Publication: Darmstadt
Year of primary publication: 2022
Publisher: Frontiers
Journal or Publication Title: Frontiers in Bioengineering and Biotechnology
Volume of the journal: 10
Collation: 10 Seiten
DOI: 10.26083/tuprints-00021336
Corresponding Links:
Origin: Secondary publication via sponsored Golden Open Access
Abstract:

Yeast-surface display (YSD) is commonly applied to screen Fab immune or naïve libraries for binders of predefined target molecules. However, reformatting of isolated variants represents a time-intensive bottleneck. Herein, we present a novel approach to facilitate a lean transition from antibody screening using YSD Fab libraries to the production of full-length IgG antibodies in Expi293-F cells. In this study, utilizing Golden Gate Cloning (GGC) and a bidirectional promoter system, an exemplary Fab-displaying YSD library was generated based on immunised transgene rats. After subsequent screening for antigen-specific antibody candidates by fluorescence-activated cell sorting (FACS), the Fab-encoding genes were subcloned into a bidirectional mammalian expression vector, exhibiting CH2-CH3 encoding genes, in a GGC-mediated, PCR-free manner. This novel, straightforward and time-saving workflow allows the VH/VL pairing to be preserved. This study resulted in antibody variants exhibiting suitable biophysical properties and covered a broad VH diversity after two rounds of FACS screening, as revealed by NGS analysis. Ultimately, we demonstrate that the implication of such a gene transfer system streamlines antibody hit discovery efforts, allowing the faster characterisation of antibodies against a plethora of targets that may lead to new therapeutic agents.

Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-213365
Additional Information:

Keywords: antibody hit discovery, bidirectional promoter, reformatting, golden gate cloning, monoclonal antibodies, yeast surface display

Classification DDC: 500 Science and mathematics > 570 Life sciences, biology
Divisions: 07 Department of Chemistry > Clemens-Schöpf-Institut > Fachgebiet Biochemie
Date Deposited: 20 May 2022 13:06
Last Modified: 14 Nov 2023 19:04
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/21336
PPN: 49489380X
Export:
Actions (login required)
View Item View Item