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A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae

Kukovetz, Kerri ; Hertel, Brigitte ; Schvarcz, Christopher R. ; Saponaro, Andrea ; Manthey, Mirja ; Burk, Ulrike ; Greiner, Timo ; Steward, Grieg F. ; Van Etten, James L. ; Moroni, Anna ; Thiel, Gerhard ; Rauh, Oliver (2021)
A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae.
In: Viruses, 2020, 12 (10)
doi: 10.26083/tuprints-00019251
Article, Secondary publication, Publisher's Version

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Item Type: Article
Type of entry: Secondary publication
Title: A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae
Language: English
Date: 2021
Year of primary publication: 2020
Publisher: MDPI
Journal or Publication Title: Viruses
Volume of the journal: 12
Issue Number: 10
Collation: 14 Seiten
DOI: 10.26083/tuprints-00019251
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Origin: Secondary publication via sponsored Golden Open Access
Abstract:

Potassium ion (K+) channels have been observed in diverse viruses that infect eukaryotic marine and freshwater algae. However, experimental evidence for functional K+ channels among these alga-infecting viruses has thus far been restricted to members of the family Phycodnaviridae, which are large, double-stranded DNA viruses within the phylum Nucleocytoviricota. Recent sequencing projects revealed that alga-infecting members of Mimiviridae, another family within this phylum, may also contain genes encoding K+ channels. Here we examine the structural features and the functional properties of putative K+ channels from four cultivated members of Mimiviridae. While all four proteins contain variations of the conserved selectivity filter sequence of K+ channels, structural prediction algorithms suggest that only two of them have the required number and position of two transmembrane domains that are present in all K+ channels. After in vitro translation and reconstitution of the four proteins in planar lipid bilayers, we confirmed that one of them, a 79 amino acid protein from the virus Tetraselmis virus 1 (TetV-1), forms a functional ion channel with a distinct selectivity for K+ over Na+ and a sensitivity to Ba2+. Thus, virus-encoded K+ channels are not limited to Phycodnaviridae but also occur in the members of Mimiviridae. The large sequence diversity among the viral K+ channels implies multiple events of lateral gene transfer.

Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-192518
Classification DDC: 500 Science and mathematics > 570 Life sciences, biology
Divisions: 10 Department of Biology > Plant Membrane Biophyscis (20.12.23 renamed in Biology of Algae and Protozoa)
Date Deposited: 03 Aug 2021 07:28
Last Modified: 03 Aug 2021 07:28
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/19251
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