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ATM Release at Resected Double-Strand Breaks Provides Heterochromatin Reconstitution to Facilitate Homologous Recombination

Geuting, Verena ; Reul, Christian ; Löbrich, Markus (2021)
ATM Release at Resected Double-Strand Breaks Provides Heterochromatin Reconstitution to Facilitate Homologous Recombination.
In: PLOS Genetics, 2013, 9 (8)
doi: 10.26083/tuprints-00019033
Article, Secondary publication, Publisher's Version

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Item Type: Article
Type of entry: Secondary publication
Title: ATM Release at Resected Double-Strand Breaks Provides Heterochromatin Reconstitution to Facilitate Homologous Recombination
Language: English
Date: 2021
Place of Publication: Darmstadt
Year of primary publication: 2013
Publisher: PLOS
Journal or Publication Title: PLOS Genetics
Volume of the journal: 9
Issue Number: 8
Collation: 14 Seiten
DOI: 10.26083/tuprints-00019033
Corresponding Links:
Origin: Secondary publication service
Abstract:

Non-homologous end-joining (NHEJ) and homologous recombination (HR) represent the two main pathways for repairing DNA double-strand breaks (DSBs). During the G2 phase of the mammalian cell cycle, both processes can operate and chromatin structure is one important factor which determines DSB repair pathway choice. ATM facilitates the repair of heterochromatic DSBs by phosphorylating and inactivating the heterochromatin building factor KAP-1, leading to local chromatin relaxation. Here, we show that ATM accumulation and activity is strongly diminished at DSBs undergoing end-resection during HR. Such DSBs remain unrepaired in cells devoid of the HR factors BRCA2, XRCC3 or RAD51. Strikingly, depletion of KAP-1 or expression of phospho-mimic KAP-1 allows repair of resected DSBs in the absence of BRCA2, XRCC3 or RAD51 by an erroneous PARP-dependent alt-NHEJ process. We suggest that DSBs in heterochromatin elicit initial local heterochromatin relaxation which is reversed during HR due to the release of ATM from resection break ends. The restored heterochromatic structure facilitates HR and prevents usage of error-prone alternative processes.

Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-190332
Classification DDC: 500 Science and mathematics > 570 Life sciences, biology
Divisions: 10 Department of Biology > Radiation Biology and DNA Repair
Date Deposited: 18 Aug 2021 12:05
Last Modified: 07 Aug 2023 10:49
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/19033
PPN: 510357970
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