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Ionizing Radiation Induces Morphological Changes and Immunological Modulation of Jurkat Cells

Voos, Patrick ; Fuck, Sebastian ; Weipert, Fabian ; Babel, Laura ; Tandl, Dominique ; Meckel, Tobias ; Hehlgans, Stephanie ; Fournier, Claudia ; Moroni, Anna ; Rödel, Franz ; Thiel, Gerhard (2018):
Ionizing Radiation Induces Morphological Changes and Immunological Modulation of Jurkat Cells.
9, In: Frontiers in Immunology, ISSN 1664-3224,
DOI: 10.3389/fimmu.2018.00922,
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Item Type: Article
Origin: Secondary publication via sponsored Golden Open Access
Title: Ionizing Radiation Induces Morphological Changes and Immunological Modulation of Jurkat Cells
Language: English
Abstract:

Impairment or stimulation of the immune system by ionizing radiation (IR) impacts on immune surveillance of tumor cells and non-malignant cells and can either foster therapy response or side effects/toxicities of radiation therapy. For a better understanding of the mechanisms by which IR modulates T-cell activation and alters functional properties of these immune cells, we exposed human immortalized Jurkat cells and peripheral blood lymphocytes (PBL) to X-ray doses between 0.1 and 5 Gy. This resulted in cellular responses, which are typically observed also in naïve T-lymphocytes in response of T-cell receptor immune stimulation or mitogens. These responses include oscillations of cytosolic Ca2+, an upregulation of CD25 surface expression, interleukin-2 and interferon-γ synthesis, elevated expression of Ca2+ sensitive K+ channels and an increase in cell diameter. The latter was sensitive to inhibition by the immunosuppressant cyclosporine A, Ca2+ buffer BAPTA-AM, and the CDK1-inhibitor RO3306, indicating the involvement of Ca2+-dependent immune activation and radiation-induced cell cycle arrest. Furthermore, on a functional level, Jurkat and PBL cell adhesion to endothelial cells was increased upon radiation exposure and was highly dependent on an upregulation of integrin beta-1 expression and clustering. In conclusion, we here report that IR impacts on immune activation and functional properties of T-lymphocytes that may have implications in both toxic effects and treatment response to combined radiation and immune therapy in cancer patients.

Journal or Publication Title: Frontiers in Immunology
Series Volume: 9
Classification DDC: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie
Divisions: 10 Department of Biology
Date Deposited: 28 May 2018 12:11
Last Modified: 09 Jul 2020 02:07
DOI: 10.3389/fimmu.2018.00922
URN: urn:nbn:de:tuda-tuprints-74493
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/7449
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