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Sensing Levofloxacin with an RNA Aptamer as a Bioreceptor

Kramat, Janice ; Kraus, Leon ; Gunawan, Vincent J. ; Smyej, Elias ; Froehlich, Philipp ; Weber, Tim E. ; Spiehl, Dieter ; Koeppl, Heinz ; Blaeser, Andreas ; Suess, Beatrix (2024)
Sensing Levofloxacin with an RNA Aptamer as a Bioreceptor.
In: Biosensors, 2024, 14 (1)
doi: 10.26083/tuprints-00027255
Article, Secondary publication, Publisher's Version

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Item Type: Article
Type of entry: Secondary publication
Title: Sensing Levofloxacin with an RNA Aptamer as a Bioreceptor
Language: English
Date: 7 May 2024
Place of Publication: Darmstadt
Year of primary publication: 22 January 2024
Place of primary publication: Basel
Publisher: MDPI
Journal or Publication Title: Biosensors
Volume of the journal: 14
Issue Number: 1
Collation: 17 Seiten
DOI: 10.26083/tuprints-00027255
Corresponding Links:
Origin: Secondary publication DeepGreen

To combat the growing threat of antibiotic resistance, environmental testing for antibiotic contamination is gaining an increasing role. This study aims to develop an easy-to-use assay for the detection of the fluoroquinolone antibiotic levofloxacin. Levofloxacin is used in human and veterinary medicine and has been detected in wastewater and river water. An RNA aptamer against levofloxacin was selected using RNA Capture-SELEX. The 73 nt long aptamer folds into three stems with a central three-way junction. It binds levofloxacin with a Kd of 6 µM and discriminates the closely related compound ciprofloxacin. Furthermore, the selection process was analyzed using a next-generation sequencing approach to better understand the sequence evolution throughout the selection. The aptamer was used as a bioreceptor for the development of a lateral flow assay. The biosensor exploited the innate characteristic of RNA Capture-SELEX to select aptamers that displace a complementary DNA oligonucleotide upon ligand binding. The lateral flow assay achieved a limit of visual detection of 100 µM. While the sensitivity of this assay constrains its immediate use in environmental testing, the present study can serve as a template for the selection of RNA aptamer-based biosensors.

Uncontrolled Keywords: aptamers, SELEX, antibiotics, levofloxacin, lateral flow assay, biosensor
Identification Number: Artikel-ID: 56
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-272551
Additional Information:

This article belongs to the Section Biosensors and Healthcare

Classification DDC: 500 Science and mathematics > 570 Life sciences, biology
600 Technology, medicine, applied sciences > 620 Engineering and machine engineering
600 Technology, medicine, applied sciences > 621.3 Electrical engineering, electronics
Divisions: 10 Department of Biology > Synthetic RNA biology
16 Department of Mechanical Engineering > Institute of Printing Science and Technology (IDD) > Biomedical Printing Technology (BMT)
18 Department of Electrical Engineering and Information Technology > Self-Organizing Systems Lab
Interdisziplinäre Forschungsprojekte > Centre for Synthetic Biology
Date Deposited: 07 May 2024 12:46
Last Modified: 17 May 2024 12:49
SWORD Depositor: Deep Green
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/27255
PPN: 518208869
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