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Respiration-based investigation of adsorbent-bioprocess compatibility

Pastoors, Johannes ; Baltin, Chris ; Bettmer, Jens ; Deitert, Alexander ; Götzen, Tobias ; Michel, Carina ; Deischter, Jeff ; Schroll, Isabel ; Biselli, Andreas ; Palkovits, Regina ; Rose, Marcus ; Jupke, Andreas ; Büchs, Jochen (2023)
Respiration-based investigation of adsorbent-bioprocess compatibility.
In: Biotechnology for Biofuels and Bioproducts, 2023, 16 (1)
doi: 10.26083/tuprints-00024527
Article, Secondary publication, Publisher's Version

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Item Type: Article
Type of entry: Secondary publication
Title: Respiration-based investigation of adsorbent-bioprocess compatibility
Language: English
Date: 20 November 2023
Place of Publication: Darmstadt
Year of primary publication: 18 March 2023
Place of primary publication: London
Publisher: Springer Nature
Journal or Publication Title: Biotechnology for Biofuels and Bioproducts
Volume of the journal: 16
Issue Number: 1
Collation: 12 Seiten
DOI: 10.26083/tuprints-00024527
Corresponding Links:
Origin: Secondary publication service

Background: The efficiency of downstream processes plays a crucial role in the transition from conventional petrochemical processes to sustainable biotechnological production routes. One promising candidate for product separation from fermentations with low energy demand and high selectivity is the adsorption of the target product on hydrophobic adsorbents. However, only limited knowledge exists about the interaction of these adsorbents and the bioprocess. The bioprocess could possibly be harmed by the release of inhibitory components from the adsorbent surface. Another possibility is co-adsorption of essential nutrients, especially in an in situ application, making these nutrients unavailable to the applied microorganism.

Results: A test protocol investigating adsorbent-bioprocess compatibility was designed and applied on a variety of adsorbents. Inhibitor release and nutrient adsorption was studied in an isolated manner. Respiratory data recorded by a RAMOS device was used to assess the influence of the adsorbents on the cultivation in three different microbial systems for up to six different adsorbents per system. While no inhibitor release was detected in our investigations, adsorption of different essential nutrients was observed.

Conclusion: The application of adsorption for product recovery from the bioprocess was proven to be generally possible, but nutrient adsorption has to be assessed for each application individually. To account for nutrient adsorption, adsorptive product separation should only be applied after sufficient microbial growth. Moreover, concentrations of co-adsorbed nutrients need to be increased to compensate nutrient loss. The presented protocol enables an investigation of adsorbent-bioprocess compatibility with high-throughput and limited effort.

Uncontrolled Keywords: Adsorption, RAMOS, Downstream processing, Integrated bioprocesses
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-245275
Classification DDC: 500 Science and mathematics > 540 Chemistry
Divisions: 07 Department of Chemistry > Ernst-Berl-Institut > Fachgebiet Technische Chemie > Technische Chemie II
Date Deposited: 20 Nov 2023 11:17
Last Modified: 27 Nov 2023 07:15
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/24527
PPN: 513465014
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