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Towards single-cell pulsed EPR using VCO-based EPR-on-a-chip detectors

Hassan, Mohamed Atef ; Kern, Michal ; Chu, Anh ; Kalra, Gatik ; Shabratova, Ekaterina ; Tsarapkin, Aleksei ; MacKinnon, Neil ; Lips, Klaus ; Teutloff, Christian ; Bittl, Robert ; Korvink, Jan Gerrit ; Anders, Jens (2023):
Towards single-cell pulsed EPR using VCO-based EPR-on-a-chip detectors. (Publisher's Version)
In: Frequenz, 76 (11-12), pp. 699-717. De Gruyter, ISSN 0016-1136,
DOI: 10.26083/tuprints-00023215,

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Item Type: Article
Origin: Secondary publication
Status: Publisher's Version
Title: Towards single-cell pulsed EPR using VCO-based EPR-on-a-chip detectors
Language: English

Electron paramagnetic resonance (EPR) is the gold standard for studying paramagnetic species. As an example, in structural biology, it allows to extract information about distance distributions on the nanometer scale via site-directed spin labeling. Conventional pulsed EPR of biological samples is currently limited to relatively large sample concentrations and cryogenic temperatures, mainly due to low sensitivity and the significant dead time associated with conventional resonator-based EPR setups, essentially precluding in-cell EPR under physiological conditions. This paper presents our latest progress toward single-cell pulsed EPR using VCO-based EPR-on-a-chip (EPRoC) sensors. Together with an analytical model for VCO-based pulsed EPR, we present an experimental scheme to perform dead time- free pulsed EPR measurements using EPRoC detectors. The proposed scheme is validated using extensive numerical simulations and proof-of-concept experiments on the spin dynamics of an organic radical at room temperature using a custom-designed EPRoC detector operating in the Ka-band around 30.4 GHz. Additionally, we discuss methods to improve the excitation field homogeneity and sample handling through chip post-processing and custom-designed microfluidics. Finally, we present our progress towards compact, portable pulsed EPR spectrometers incorporating EPRoC detectors, microfluidics, and custom-designed permanent magnets. Such portable EPR spectrometers can pave the way toward new EPR applications, including point-of care diagnostics.

Journal or Publication Title: Frequenz
Volume of the journal: 76
Issue Number: 11-12
Place of Publication: Darmstadt
Publisher: De Gruyter
Uncontrolled Keywords: dead time free EPR, EPR, EPR-on-a-chip, EPRoC, Rabi oscillations, VCO-based pulsed EPR
Classification DDC: 500 Naturwissenschaften und Mathematik > 530 Physik
500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie
600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften und Maschinenbau
Divisions: 18 Department of Electrical Engineering and Information Technology > Institute for Microwave Engineering and Photonics (IMP)
Date Deposited: 01 Mar 2023 10:02
Last Modified: 01 Mar 2023 10:02
DOI: 10.26083/tuprints-00023215
Corresponding Links:
URN: urn:nbn:de:tuda-tuprints-232151
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/23215
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