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Towards single-cell pulsed EPR using VCO-based EPR-on-a-chip detectors

Hassan, Mohamed Atef ; Kern, Michal ; Chu, Anh ; Kalra, Gatik ; Shabratova, Ekaterina ; Tsarapkin, Aleksei ; MacKinnon, Neil ; Lips, Klaus ; Teutloff, Christian ; Bittl, Robert ; Korvink, Jan Gerrit ; Anders, Jens (2023)
Towards single-cell pulsed EPR using VCO-based EPR-on-a-chip detectors.
In: Frequenz, 2022, 76 (11-12)
doi: 10.26083/tuprints-00023215
Article, Secondary publication, Publisher's Version

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Item Type: Article
Type of entry: Secondary publication
Title: Towards single-cell pulsed EPR using VCO-based EPR-on-a-chip detectors
Language: English
Date: 1 March 2023
Place of Publication: Darmstadt
Year of primary publication: 2022
Publisher: De Gruyter
Journal or Publication Title: Frequenz
Volume of the journal: 76
Issue Number: 11-12
DOI: 10.26083/tuprints-00023215
Corresponding Links:
Origin: Secondary publication

Electron paramagnetic resonance (EPR) is the gold standard for studying paramagnetic species. As an example, in structural biology, it allows to extract information about distance distributions on the nanometer scale via site-directed spin labeling. Conventional pulsed EPR of biological samples is currently limited to relatively large sample concentrations and cryogenic temperatures, mainly due to low sensitivity and the significant dead time associated with conventional resonator-based EPR setups, essentially precluding in-cell EPR under physiological conditions. This paper presents our latest progress toward single-cell pulsed EPR using VCO-based EPR-on-a-chip (EPRoC) sensors. Together with an analytical model for VCO-based pulsed EPR, we present an experimental scheme to perform dead time- free pulsed EPR measurements using EPRoC detectors. The proposed scheme is validated using extensive numerical simulations and proof-of-concept experiments on the spin dynamics of an organic radical at room temperature using a custom-designed EPRoC detector operating in the Ka-band around 30.4 GHz. Additionally, we discuss methods to improve the excitation field homogeneity and sample handling through chip post-processing and custom-designed microfluidics. Finally, we present our progress towards compact, portable pulsed EPR spectrometers incorporating EPRoC detectors, microfluidics, and custom-designed permanent magnets. Such portable EPR spectrometers can pave the way toward new EPR applications, including point-of care diagnostics.

Uncontrolled Keywords: dead time free EPR, EPR, EPR-on-a-chip, EPRoC, Rabi oscillations, VCO-based pulsed EPR
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-232151
Classification DDC: 500 Science and mathematics > 530 Physics
500 Science and mathematics > 570 Life sciences, biology
600 Technology, medicine, applied sciences > 620 Engineering and machine engineering
Divisions: 18 Department of Electrical Engineering and Information Technology > Institute for Microwave Engineering and Photonics (IMP)
Date Deposited: 01 Mar 2023 10:02
Last Modified: 29 Sep 2023 07:41
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/23215
PPN: 511954298
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