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Evaluation of a quick one‐step sample preparation method for the determination of the isotopic fingerprint of rapeseed (Brassica napus): Investigation of the influence of the use of 2,2‐dimethoxypropane on compound‐specific stable carbon and hydrogen isotope analyses by gas chromatography combustion/pyrolysis isotope ratio mass spectrometry

Xia, Lili ; Sakaguchi‐Söder, Kaori ; Stanojkovski, Daniel ; Schebek, Liselotte (2022)
Evaluation of a quick one‐step sample preparation method for the determination of the isotopic fingerprint of rapeseed (Brassica napus): Investigation of the influence of the use of 2,2‐dimethoxypropane on compound‐specific stable carbon and hydrogen isotope analyses by gas chromatography combustion/pyrolysis isotope ratio mass spectrometry.
In: Rapid Communications in Mass Spectrometry, 2021, 35 (9)
doi: 10.26083/tuprints-00023026
Article, Secondary publication, Publisher's Version

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Item Type: Article
Type of entry: Secondary publication
Title: Evaluation of a quick one‐step sample preparation method for the determination of the isotopic fingerprint of rapeseed (Brassica napus): Investigation of the influence of the use of 2,2‐dimethoxypropane on compound‐specific stable carbon and hydrogen isotope analyses by gas chromatography combustion/pyrolysis isotope ratio mass spectrometry
Language: English
Date: 20 December 2022
Place of Publication: Darmstadt
Year of primary publication: 2021
Publisher: Wiley
Journal or Publication Title: Rapid Communications in Mass Spectrometry
Volume of the journal: 35
Issue Number: 9
Collation: 11 Seiten
DOI: 10.26083/tuprints-00023026
Corresponding Links:
Origin: Secondary publication service
Abstract:

Rationale

Gas chromatographic analyses for vegetable oils require transesterification, which generally involves multiple steps, mainly to generate fatty acid methyl esters (FAMEs). A quick method based on acid-catalyzed transesterification using 2,2-dimethoxypropane (DMP) enables the conversion in one step, in a single reactor. For compound-specific stable carbon and hydrogen isotope analyses (C- and H-CSIA) of individual fatty acids (FAs) in oil, the verification of this one-step method has not yet been reported.

Methods

In this study, we evaluated the feasibility of the one-step method for C- and H-CSIA of individual FAMEs in rapeseed samples. The focus was on the investigation of the influence of methanol, which was produced from the reactions of DMP with glycerol and water during transesterification, on the accuracy of isotope composition of FAMEs, consequently of the FAs. The reproducibility of the one-step method was assessed by the measurement of the FAMEs from rapeseed and rapeseed oil. For the C- and H-CSIA of individual FAMEs, a gas chromatography combustion/pyrolysis isotope ratio mass spectrometry system was used.

Results

Our results showed that no significant differences arise in the carbon and hydrogen isotope compositions of the selected main FAMEs produced with and without DMP except for the H-CSIA value of C18:3. The reproducibility of the one-step method for rapeseed was in the range of ±0.1 mUr to ± 0.3 mUr for C-CSIA and ±1 mUr to ±3 mUr for H-CSIA of the main FAMEs.

Identification Number: Artikel-ID: e9064
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-230260
Additional Information:

Peer review history: https://t1p.de/l8z62

Classification DDC: 600 Technology, medicine, applied sciences > 620 Engineering and machine engineering
Divisions: 13 Department of Civil and Environmental Engineering Sciences > Institute IWAR > Material Flow Management and Resource Economy
Date Deposited: 20 Dec 2022 13:04
Last Modified: 26 Jul 2024 08:56
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/23026
PPN: 507666186
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