Ali, Ataurehman ; Happel, Dominic ; Habermann, Jan ; Schoenfeld, Katrin ; Macarrón Palacios, Arturo ; Bitsch, Sebastian ; Englert, Simon ; Schneider, Hendrik ; Avrutina, Olga ; Fabritz, Sebastian ; Kolmar, Harald (2022):
Sactipeptide Engineering by Probing the Substrate Tolerance of a Thioether‐Bond‐Forming Sactisynthase. (Publisher's Version)
In: Angewandte Chemie International Edition, 61 (45), Wiley-VCH, e-ISSN 1521-3773,
DOI: 10.26083/tuprints-00022904,
[Article]
![]() |
Text
ANIE_ANIE202210883.pdf Copyright Information: CC-BY-NC 4.0 International - Creative Commons, Attribution NonCommercial. Download (7MB) |
![]() |
Text
(Supplement)
anie202210883-sup-0001-misc_information.pdf Copyright Information: CC-BY-NC 4.0 International - Creative Commons, Attribution NonCommercial. Download (4MB) |
Item Type: | Article |
---|---|
Origin: | Secondary publication DeepGreen |
Status: | Publisher's Version |
Title: | Sactipeptide Engineering by Probing the Substrate Tolerance of a Thioether‐Bond‐Forming Sactisynthase |
Language: | English |
Abstract: | Sactipeptides are ribosomally synthesized peptides containing a unique sulfur to α‐carbon crosslink. Catalyzed by sactisynthases, this thioether pattern endows sactipeptides with enhanced structural, thermal, and proteolytic stability, which makes them attractive scaffolds for the development of novel biotherapeutics. Herein, we report the in‐depth study on the substrate tolerance of the sactisynthase AlbA to catalyze the formation of thioether bridges in sactipeptides. We identified a possible modification site within the sactipeptide subtilosin A allowing for peptide engineering without compromising formation of thioether bridges. A panel of natural and hybrid sactipeptides was produced to study the AlbA‐mediated formation of thioether bridges, which were identified mass‐spectrometrically. In a proof‐of‐principle study, we re‐engineered subtilosin A to a thioether‐bridged, specific streptavidin targeting peptide, opening the door for the functional engineering of sactipeptides. |
Journal or Publication Title: | Angewandte Chemie International Edition |
Volume of the journal: | 61 |
Issue Number: | 45 |
Place of Publication: | Darmstadt |
Publisher: | Wiley-VCH |
Collation: | 8 Seiten |
Uncontrolled Keywords: | Bioengineering, Miniproteins, RiPPs, Sactipeptides, Sactisynthases |
Classification DDC: | 500 Naturwissenschaften und Mathematik > 540 Chemie 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie |
Divisions: | Interdisziplinäre Forschungsprojekte > Centre for Synthetic Biology 07 Department of Chemistry > Fachgebiet Biochemie |
Date Deposited: | 23 Dec 2022 14:16 |
Last Modified: | 04 Jan 2023 08:07 |
DOI: | 10.26083/tuprints-00022904 |
Corresponding Links: | |
URN: | urn:nbn:de:tuda-tuprints-229041 |
Additional Information: | International Version |
SWORD Depositor: | Deep Green |
URI: | https://tuprints.ulb.tu-darmstadt.de/id/eprint/22904 |
PPN: | 503250910 |
Export: |
![]() |
View Item |