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DNA Penetration into a Lysozyme Layer at the Surface of Aqueous Solutions

Chirkov, Nikolay S. ; Lin, Shi-Yow ; Michailov, Alexander V. ; Miller, Reinhard ; Noskov, Boris A. (2022)
DNA Penetration into a Lysozyme Layer at the Surface of Aqueous Solutions.
In: International Journal of Molecular Sciences, 2022, 23 (20)
doi: 10.26083/tuprints-00022834
Article, Secondary publication, Publisher's Version

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Item Type: Article
Type of entry: Secondary publication
Title: DNA Penetration into a Lysozyme Layer at the Surface of Aqueous Solutions
Language: English
Date: 7 November 2022
Place of Publication: Darmstadt
Year of primary publication: 2022
Publisher: MDPI
Journal or Publication Title: International Journal of Molecular Sciences
Volume of the journal: 23
Issue Number: 20
Collation: 12 Seiten
DOI: 10.26083/tuprints-00022834
Corresponding Links:
Origin: Secondary publication DeepGreen

The interactions of DNA with lysozyme in the surface layer were studied by performing infrared reflection–absorption spectroscopy (IRRAS), ellipsometry, surface tensiometry, surface dilational rheology, and atomic force microscopy (AFM). A concentrated DNA solution was injected into an aqueous subphase underneath a spread lysozyme layer. While the optical properties of the surface layer changed fast after DNA injection, the dynamic dilational surface elasticity almost did not change, thereby indicating no continuous network formation of DNA/lysozyme complexes, unlike the case of DNA interactions with a monolayer of a cationic synthetic polyelectrolyte. A relatively fast increase in optical signals after a DNA injection under a lysozyme layer indicates that DNA penetration is controlled by diffusion. At low surface pressures, the AFM images show the formation of long strands in the surface layer. Increased surface compression does not lead to the formation of a network of DNA/lysozyme aggregates as in the case of a mixed layer of DNA and synthetic polyelectrolytes, but to the appearance of some folds and ridges in the layer. The formation of more disordered aggregates is presumably a consequence of weaker interactions of lysozyme with duplex DNA and the stabilization, at the same time, of loops of unpaired nucleotides at high local lysozyme concentrations in the surface layer.

Uncontrolled Keywords: DNA, lysozyme, adsorption kinetics, dilational surface rheology, Langmuir monolayers
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-228345
Additional Information:

This article belongs to the Special Issue The Self-Assembly and Design of Polyfunctional Nanosystems 3.0

Classification DDC: 500 Science and mathematics > 530 Physics
500 Science and mathematics > 540 Chemistry
Divisions: 05 Department of Physics > Institute for Condensed Matter Physics > Soft Matter Biophysics
Date Deposited: 07 Nov 2022 12:05
Last Modified: 14 Nov 2023 19:05
SWORD Depositor: Deep Green
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/22834
PPN: 501612750
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