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Specific and conserved patterns of microbiota-structuring by maize benzoxazinoids in the field

Cadot, Selma ; Guan, Hang ; Bigalke, Moritz ; Walser, Jean-Claude ; Jander, Georg ; Erb, Matthias ; Heijden, Marcel G. A. van der ; Schlaeppi, Klaus (2022)
Specific and conserved patterns of microbiota-structuring by maize benzoxazinoids in the field.
In: Microbiome, 9
doi: 10.26083/tuprints-00022543
Article, Secondary publication, Publisher's Version

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Item Type: Article
Type of entry: Secondary publication
Title: Specific and conserved patterns of microbiota-structuring by maize benzoxazinoids in the field
Language: English
Date: 2022
Place of Publication: Darmstadt
Publisher: Springer BMC
Journal or Publication Title: Microbiome
Volume of the journal: 9
Collation: 19 Seiten
DOI: 10.26083/tuprints-00022543
Corresponding Links:
Origin: Secondary publication service


Plants influence their root and rhizosphere microbial communities through the secretion of root exudates. However, how specific classes of root exudate compounds impact the assembly of root-associated microbiotas is not well understood, especially not under realistic field conditions. Maize roots secrete benzoxazinoids (BXs), a class of indole-derived defense compounds, and thereby impact the assembly of their microbiota. Here, we investigated the broader impacts of BX exudation on root and rhizosphere microbiotas of adult maize plants grown under natural conditions at different field locations in Europe and the USA. We examined the microbiotas of BX-producing and multiple BX-defective lines in two genetic backgrounds across three soils with different properties.


Our analysis showed that BX secretion affected the community composition of the rhizosphere and root microbiota, with the most pronounced effects observed for root fungi. The impact of BX exudation was at least as strong as the genetic background, suggesting that BX exudation is a key trait by which maize structures its associated microbiota. BX-producing plants were not consistently enriching microbial lineages across the three field experiments. However, BX exudation consistently depleted Flavobacteriaceae and Comamonadaceae and enriched various potential plant pathogenic fungi in the roots across the different environments.


These findings reveal that BXs have a selective impact on root and rhizosphere microbiota composition across different conditions. Taken together, this study identifies the BX pathway as an interesting breeding target to manipulate plant-microbiome interactions.

Uncontrolled Keywords: Zea mays, Root exudates, Benzoxazinoids, Rhizosphere, Root microbiota
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-225436
Additional Information:

Github project: https://github.com/PMI-Basel/Cadot_et_al_BX_microbiota

Classification DDC: 500 Science and mathematics > 550 Earth sciences and geology
Divisions: 11 Department of Materials and Earth Sciences > Earth Science > Department of Soil Mineralogy and Soil Chemistry
Date Deposited: 14 Dec 2022 13:54
Last Modified: 28 Apr 2023 07:48
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/22543
PPN: 507310683
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