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3D-Printed PLA-Bioglass Scaffolds with Controllable Calcium Release and MSC Adhesion for Bone Tissue Engineering

Schätzlein, Eva ; Kicker, Christoph ; Söhling, Nicolas ; Ritz, Ulrike ; Neijhoft, Jonas ; Henrich, Dirk ; Frank, Johannes ; Marzi, Ingo ; Blaeser, Andreas (2022):
3D-Printed PLA-Bioglass Scaffolds with Controllable Calcium Release and MSC Adhesion for Bone Tissue Engineering. (Publisher's Version)
In: Polymers, 14 (12), MDPI, e-ISSN 2073-4360,
DOI: 10.26083/tuprints-00021633,
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Item Type: Article
Origin: Secondary publication DeepGreen
Status: Publisher's Version
Title: 3D-Printed PLA-Bioglass Scaffolds with Controllable Calcium Release and MSC Adhesion for Bone Tissue Engineering
Language: English
Abstract:

Large bone defects are commonly treated by replacement with auto- and allografts, which have substantial drawbacks including limited supply, donor site morbidity, and possible tissue rejection. This study aimed to improve bone defect treatment using a custom-made filament for tissue engineering scaffolds. The filament consists of biodegradable polylactide acid (PLA) and a varying amount (up to 20%) of osteoconductive S53P4 bioglass. By employing an innovative, additive manufacturing technique, scaffolds with optimized physico-mechanical and biological properties were produced. The scaffolds feature adjustable macro- and microporosity (200–2000 µm) with adaptable mechanical properties (83–135 MPa). Additionally, controllable calcium release kinetics (0–0.25 nMol/µL after 24 h), tunable mesenchymal stem cell (MSC) adhesion potential (after 24 h by a factor of 14), and proliferation (after 168 h by a factor of 18) were attained. Microgrooves resulting from the 3D-printing process on the surface act as a nucleus for cell aggregation, thus being a potential cell niche for spheroid formation or possible cell guidance. The scaffold design with its adjustable biomechanics and the bioglass with its antimicrobial properties are of particular importance for the preclinical translation of the results. This study comprehensibly demonstrates the potential of a 3D-printed bioglass composite scaffold for the treatment of critical-sized bone defects.

Journal or Publication Title: Polymers
Volume of the journal: 14
Issue Number: 12
Place of Publication: Darmstadt
Publisher: MDPI
Collation: 15 Seiten
Uncontrolled Keywords: bone tissue engineering, cell seeding, biofabrication, fused filament fabrication, 3D printing, bioactive glass, polymer ceramic composites, PLA bioglass
Classification DDC: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie
600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften
Divisions: 16 Department of Mechanical Engineering > Institute of Printing Science and Technology (IDD) > Biomedical Printing Technology (BMT)
Interdisziplinäre Forschungsprojekte > Centre for Synthetic Biology
Date Deposited: 11 Jul 2022 13:29
Last Modified: 06 Sep 2022 10:41
DOI: 10.26083/tuprints-00021633
Corresponding Links:
URN: urn:nbn:de:tuda-tuprints-216333
SWORD Depositor: Deep Green
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/21633
PPN: 498913627
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