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Rapid Capture of Cancer Extracellular Vesicles by Lipid Patch Microarrays

Liu, Hui‐Yu ; Kumar, Ravi ; Zhong, Chunting ; Gorji, Saleh ; Paniushkina, Liliia ; Masood, Ramsha ; Wittel, Uwe A. ; Fuchs, Harald ; Nazarenko, Irina ; Hirtz, Michael (2024)
Rapid Capture of Cancer Extracellular Vesicles by Lipid Patch Microarrays.
In: Advanced Materials, 2021, 33 (35)
doi: 10.26083/tuprints-00021002
Article, Secondary publication, Publisher's Version

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Item Type: Article
Type of entry: Secondary publication
Title: Rapid Capture of Cancer Extracellular Vesicles by Lipid Patch Microarrays
Language: English
Date: 13 February 2024
Place of Publication: Darmstadt
Year of primary publication: 2021
Place of primary publication: Weinheim
Publisher: Wiley-VCH
Journal or Publication Title: Advanced Materials
Volume of the journal: 33
Issue Number: 35
Collation: 13 Seiten
DOI: 10.26083/tuprints-00021002
Corresponding Links:
Origin: Secondary publication DeepGreen
Abstract:

Extracellular vesicles (EVs) contain various bioactive molecules such as DNA, RNA, and proteins, and play a key role in the regulation of cancer progression. Furthermore, cancer‐associated EVs carry specific biomarkers and can be used in liquid biopsy for cancer detection. However, it is still technically challenging and time consuming to detect or isolate cancer‐associated EVs from complex biofluids (e.g., blood). Here, a novel EV‐capture strategy based on dip‐pen nanolithography generated microarrays of supported lipid membranes is presented. These arrays carry specific antibodies recognizing EV‐ and cancer‐specific surface biomarkers, enabling highly selective and efficient capture. Importantly, it is shown that the nucleic acid cargo of captured EVs is retained on the lipid array, providing the potential for downstream analysis. Finally, the feasibility of EV capture from patient sera is demonstrated. The demonstrated platform offers rapid capture, high specificity, and sensitivity, with only a small need in analyte volume and without additional purification steps. The platform is applied in context of cancer‐associated EVs, but it can easily be adapted to other diagnostic EV targets by use of corresponding antibodies.

Uncontrolled Keywords: breast cancer, dip‐pen nanolithography, extracellular vesicles, scanning probe lithography, supported lipid bilayers
Identification Number: Artikel-ID: 2008493
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-210029
Classification DDC: 600 Technology, medicine, applied sciences > 600 Technology
600 Technology, medicine, applied sciences > 610 Medicine and health
600 Technology, medicine, applied sciences > 660 Chemical engineering
Divisions: 11 Department of Materials and Earth Sciences > Material Science > Joint Research Laboratory Nanomaterials
Date Deposited: 13 Feb 2024 13:44
Last Modified: 30 Apr 2024 09:44
SWORD Depositor: Deep Green
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/21002
PPN: 517426579
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