Eckert, Denise ; Rapp, Felicitas ; Tsedeke, Ayele T. ; Molendowska, Jessica ; Lehn, Robert ; Langhans, Markus ; Fournier, Claudia ; Rödel, Franz ; Hehlgans, Stephanie (2022):
ROS- and Radiation Source-Dependent Modulation of Leukocyte Adhesion to Primary Microvascular Endothelial Cells. (Publisher's Version)
In: Cells, 11 (1), MDPI, e-ISSN 2073-4409,
DOI: 10.26083/tuprints-00020277,
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Item Type: | Article |
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Origin: | Secondary publication DeepGreen |
Status: | Publisher's Version |
Title: | ROS- and Radiation Source-Dependent Modulation of Leukocyte Adhesion to Primary Microvascular Endothelial Cells |
Language: | English |
Abstract: | Anti-inflammatory effects of low-dose irradiation often follow a non-linear dose–effect relationship. These characteristics were also described for the modulation of leukocyte adhesion to endothelial cells. Previous results further revealed a contribution of reactive oxygen species (ROS) and anti-oxidative factors to a reduced leukocyte adhesion. Here, we evaluated the expression of anti-oxidative enzymes and the transcription factor Nrf2 (Nuclear factor-erythroid-2-related factor 2), intracellular ROS content, and leukocyte adhesion in primary human microvascular endothelial cells (HMVEC) upon low-dose irradiation under physiological laminar shear stress or static conditions after irradiation with X-ray or Carbon (C)-ions (0–2 Gy). Laminar conditions contributed to increased mRNA expression of anti-oxidative factors and reduced ROS in HMVEC following a 0.1 Gy X-ray and 0.5 Gy C-ion exposure, corresponding to reduced leukocyte adhesion and expression of adhesion molecules. By contrast, mRNA expression of anti-oxidative markers and adhesion molecules, ROS, and leukocyte adhesion were not altered by irradiation under static conditions. In conclusion, irradiation of endothelial cells with low doses under physiological laminar conditions modulates the mRNA expression of key factors of the anti-oxidative system, the intracellular ROS contents of which contribute at least in part to leucocyte adhesion, dependent on the radiation source. |
Journal or Publication Title: | Cells |
Volume of the journal: | 11 |
Issue Number: | 1 |
Place of Publication: | Darmstadt |
Publisher: | MDPI |
Collation: | 20 Seiten |
Uncontrolled Keywords: | adhesion, endothelial cells, inflammation, leukocytes, low-dose irradiation, shear stress |
Classification DDC: | 500 Naturwissenschaften und Mathematik > 530 Physik 500 Naturwissenschaften und Mathematik > 540 Chemie 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin, Gesundheit |
Divisions: | 10 Department of Biology > Membrane Dynamics 07 Department of Chemistry > Fachgebiet Makromolekulare Chemie 07 Department of Chemistry > Fachgebiet Technische Chemie |
Date Deposited: | 22 Apr 2022 12:17 |
Last Modified: | 21 Oct 2022 13:21 |
DOI: | 10.26083/tuprints-00020277 |
Corresponding Links: | |
URN: | urn:nbn:de:tuda-tuprints-202773 |
SWORD Depositor: | Deep Green |
URI: | https://tuprints.ulb.tu-darmstadt.de/id/eprint/20277 |
PPN: | 500472661 |
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