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ROS- and Radiation Source-Dependent Modulation of Leukocyte Adhesion to Primary Microvascular Endothelial Cells

Eckert, Denise ; Rapp, Felicitas ; Tsedeke, Ayele T. ; Molendowska, Jessica ; Lehn, Robert ; Langhans, Markus ; Fournier, Claudia ; Rödel, Franz ; Hehlgans, Stephanie (2022):
ROS- and Radiation Source-Dependent Modulation of Leukocyte Adhesion to Primary Microvascular Endothelial Cells. (Publisher's Version)
In: Cells, 11 (1), MDPI, e-ISSN 2073-4409,
DOI: 10.26083/tuprints-00020277,
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Item Type: Article
Origin: Secondary publication DeepGreen
Status: Publisher's Version
Title: ROS- and Radiation Source-Dependent Modulation of Leukocyte Adhesion to Primary Microvascular Endothelial Cells
Language: English
Abstract:

Anti-inflammatory effects of low-dose irradiation often follow a non-linear dose–effect relationship. These characteristics were also described for the modulation of leukocyte adhesion to endothelial cells. Previous results further revealed a contribution of reactive oxygen species (ROS) and anti-oxidative factors to a reduced leukocyte adhesion. Here, we evaluated the expression of anti-oxidative enzymes and the transcription factor Nrf2 (Nuclear factor-erythroid-2-related factor 2), intracellular ROS content, and leukocyte adhesion in primary human microvascular endothelial cells (HMVEC) upon low-dose irradiation under physiological laminar shear stress or static conditions after irradiation with X-ray or Carbon (C)-ions (0–2 Gy). Laminar conditions contributed to increased mRNA expression of anti-oxidative factors and reduced ROS in HMVEC following a 0.1 Gy X-ray and 0.5 Gy C-ion exposure, corresponding to reduced leukocyte adhesion and expression of adhesion molecules. By contrast, mRNA expression of anti-oxidative markers and adhesion molecules, ROS, and leukocyte adhesion were not altered by irradiation under static conditions. In conclusion, irradiation of endothelial cells with low doses under physiological laminar conditions modulates the mRNA expression of key factors of the anti-oxidative system, the intracellular ROS contents of which contribute at least in part to leucocyte adhesion, dependent on the radiation source.

Journal or Publication Title: Cells
Volume of the journal: 11
Issue Number: 1
Publisher: MDPI
Collation: 20 Seiten
Uncontrolled Keywords: adhesion, endothelial cells, inflammation, leukocytes, low-dose irradiation, shear stress
Classification DDC: 500 Naturwissenschaften und Mathematik > 530 Physik
500 Naturwissenschaften und Mathematik > 540 Chemie
500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie
600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin, Gesundheit
Divisions: 10 Department of Biology > Membrane Dynamics
07 Department of Chemistry > Fachgebiet Makromolekulare Chemie
07 Department of Chemistry > Fachgebiet Technische Chemie
Date Deposited: 22 Apr 2022 12:17
Last Modified: 22 Apr 2022 12:17
DOI: 10.26083/tuprints-00020277
Corresponding Links:
URN: urn:nbn:de:tuda-tuprints-202773
SWORD Depositor: Deep Green
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/20277
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