TU Darmstadt / ULB / TUprints

Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles

Gawlitza, Kornelia ; Wu, Changzhu ; Georgieva, Radostina ; Ansorge-Schumacher, Marion ; Klitzing, Regine von (2022):
Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles. (Publisher's Version)
In: Zeitschrift für Physikalische Chemie, 226 (7-8), pp. 749-759. De Gruyter, ISSN 0942-9352, e-ISSN 2196-7156,
DOI: 10.26083/tuprints-00019949,
[Article]

[img] Text
10.1524_zpch.2012.0271.pdf
Copyright Information: In Copyright.

Download (281kB)
Item Type: Article
Origin: Secondary publication service
Status: Publisher's Version
Title: Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles
Language: English
Abstract:

The immobilization of lipase B from Candida antarctica(CalB) within micronsized poly-N-Isopropylacrylamide (p-NIPAM) microgel particles with a crosslinker content of 5% is reported. The immobilization of the enzyme was reached by an exchange from polar to organic solvents. After determining the embedded amount of CalB within the polymer network, an enhanced specificactivity inn-hexane was obtained. Due to the thermoresponsibility of the polymer particles, the activity reaction was done at 25°C and 50°C. The results presented show that the reversiblecollapse of the microgel leads to a decreased activity with increasing temperature. Hence, p-NIPAMmicrogels display a good opportunity to tailor the activity of CalB. An interesting side effect is that CalB presents a suitable probe to estimate the mesh size of the polymer network, since itpenetrates in the unlabeled form but not after labeling with FITC.

Journal or Publication Title: Zeitschrift für Physikalische Chemie
Volume of the journal: 226
Issue Number: 7-8
Place of Publication: Darmstadt
Publisher: De Gruyter
Uncontrolled Keywords: Lipase B; p-NIPAM; Immobilization; Catalytic Activity; Thermoresponsibility
Classification DDC: 500 Naturwissenschaften und Mathematik > 530 Physik
500 Naturwissenschaften und Mathematik > 540 Chemie
Divisions: 05 Department of Physics > Institute for Condensed Matter Physics
Date Deposited: 06 Dec 2022 12:27
Last Modified: 20 Mar 2023 08:15
DOI: 10.26083/tuprints-00019949
Corresponding Links:
URN: urn:nbn:de:tuda-tuprints-199499
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/19949
PPN: 506130223
Export:
Actions (login required)
View Item View Item