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Codon Bias Can Determine Sorting of a Potassium Channel Protein

Engel, Anja J. ; Kithil, Marina ; Langhans, Markus ; Rauh, Oliver ; Cartolano, Matea ; Etten, James L. Van ; Moroni, Anna ; Thiel, Gerhard (2021)
Codon Bias Can Determine Sorting of a Potassium Channel Protein.
In: Cells, 2021, 10 (5)
doi: 10.26083/tuprints-00019352
Article, Secondary publication, Publisher's Version

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Item Type: Article
Type of entry: Secondary publication
Title: Codon Bias Can Determine Sorting of a Potassium Channel Protein
Language: English
Date: 25 August 2021
Place of Publication: Darmstadt
Year of primary publication: 2021
Publisher: MDPI
Journal or Publication Title: Cells
Volume of the journal: 10
Issue Number: 5
Collation: 17 Seiten
DOI: 10.26083/tuprints-00019352
Corresponding Links:
Origin: Secondary publication via sponsored Golden Open Access

Due to the redundancy of the genetic code most amino acids are encoded by multiple synonymous codons. It has been proposed that a biased frequency of synonymous codons can affect the function of proteins by modulating distinct steps in transcription, translation and folding. Here, we use two similar prototype K+ channels as model systems to examine whether codon choice has an impact on protein sorting. By monitoring transient expression of GFP-tagged channels in mammalian cells, we find that one of the two channels is sorted in a codon and cell cycle-dependent manner either to mitochondria or the secretory pathway. The data establish that a gene with either rare or frequent codons serves, together with a cell-state-dependent decoding mechanism, as a secondary code for sorting intracellular membrane proteins.

Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-193522
Additional Information:

Keywords: codon usage; effect of synonymous codon exchange; membrane protein sorting; dual sorting

Classification DDC: 500 Science and mathematics > 570 Life sciences, biology
Divisions: 10 Department of Biology > Plant Membrane Biophyscis (20.12.23 renamed in Biology of Algae and Protozoa)
Date Deposited: 25 Aug 2021 12:25
Last Modified: 14 Nov 2023 19:03
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/19352
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