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Sister chromatid exchanges occur in G₂-irradiated cells

Conrad, Sandro ; Künzel, Julia ; Löbrich, Markus (2021)
Sister chromatid exchanges occur in G₂-irradiated cells.
In: Cell Cycle, 2011, 10 (2)
doi: 10.26083/tuprints-00019036
Article, Secondary publication, Publisher's Version

Sister chromatid exchanges occur in G2 irradiated cells.pdf
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Item Type: Article
Type of entry: Secondary publication
Title: Sister chromatid exchanges occur in G₂-irradiated cells
Language: English
Date: 2021
Place of Publication: Darmstadt
Year of primary publication: 2011
Publisher: Taylor and Francis Group
Journal or Publication Title: Cell Cycle
Volume of the journal: 10
Issue Number: 2
DOI: 10.26083/tuprints-00019036
Corresponding Links:
Origin: Secondary publication service

DNA double-strand breaks (DSBs) are arguably the most important lesions induced by ionizing radiation (IR) since unrepaired or mis-repaired DSBs can lead to chromosomal aberrations and cell death. The two major pathways to repair IR-induced DSBs are non-homologous end-joining (NHEJ) and homologous recombination (HR). Perhaps surprisingly, NHEJ represents the predominant pathway in the G1 and G2 phases of the cell cycle, but HR also contributes and repairs a subset of IR-induced DSBs in G2. Following S-phase-dependent genotoxins, HR events give rise to sister chromatid exchanges (SCEs), which can be detected cytogenetically in mitosis. Here, we describe that HR occurring in G2-irradiated cells also generates SCEs in ~50% of HR events. Since HR of IR-induced DSBs in G2 is a slow process, SCE formation in G2-irradiated cells requires several hours. During this time, irradiated S-phase cells can also reach mitosis, which has contributed to the widely held belief that SCEs form only during S phase. We describe procedures to measure SCEs exclusively in G2-irradiated cells and provide evidence that following IR cells do not need to progress through S phase in order to form SCEs.

Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-190369
Classification DDC: 500 Science and mathematics > 570 Life sciences, biology
Divisions: 10 Department of Biology > Radiation Biology and DNA Repair
Date Deposited: 08 Sep 2021 12:09
Last Modified: 07 Aug 2023 10:55
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/19036
PPN: 510366600
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