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¹H NMR and Calorimetric Measurements on Rabbit Eye Lenses

Gutsze, Aleksander ; Bodurka, Jerzy ; Olechnowicz, Robert ; Buntkowsky, Gerd ; Limbach, Hans-Heinrich (2022):
¹H NMR and Calorimetric Measurements on Rabbit Eye Lenses. (Publisher's Version)
In: Zeitschrift für Naturforschung C, 50 (5-6), pp. 410-418. De Gruyter, ISSN 0939-5075, e-ISSN 1865-7125,
DOI: 10.26083/tuprints-00018893,
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Item Type: Article
Origin: Secondary publication service
Status: Publisher's Version
Title: ¹H NMR and Calorimetric Measurements on Rabbit Eye Lenses
Language: English
Abstract:

The dynamic properties of water molecules in the rabbit lens were studied by proton nuclear magnetic resonance line shape analysis, measurements of relaxation times as a function of temperature and calorimetric measurements. The experiments prove, as already suggested by other authors, that there are two types of water in the lens of rabbit eyes, namely bound unfreezable hydration water and bulk freezable water. Line shape analysis and relaxometry showed, that this two types of water exist in two different environments, which may be identified as the nucleus and the cortex of the lens. The line shape analysis showed furthermore that water molecules in the rabbit lens has a common spin lattice relaxation time (T₁), but two different transverse relaxation times (T₂A and T₂B). The tentative model of fast water exchange on the T₁ time scale and slow water exchange on the T₂ time scale, was used to explain experimental proton relaxation data of the rabbit lens. An estimation for this exchange rate kₑₓ by comparing it to the relaxation times is given (T₁⁻¹≪kₑₓ≪ T₁⁻¹). It has also been shown by a calorimetric measurements, that the lenses can be easily undercooled to temperatures well below the freezing point of water. The achievable maximum undercooling temperature of the lens is a function of the cooling rate KC, therefore it has to be considered as an experimentally adjustable parameter which is not characteristic for the investigated sample. Thus it must be noted that any previous discussions about the specific value of the temperature of water crystallisation in biological systems need to be carefully reconsidered.

Journal or Publication Title: Zeitschrift für Naturforschung C
Volume of the journal: 50
Issue Number: 5-6
Publisher: De Gruyter
Classification DDC: 500 Naturwissenschaften und Mathematik > 540 Chemie
Divisions: 07 Department of Chemistry > Physical Chemistry
Date Deposited: 07 Jan 2022 13:30
Last Modified: 07 Jan 2022 13:30
DOI: 10.26083/tuprints-00018893
Corresponding Links:
URN: urn:nbn:de:tuda-tuprints-188932
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/18893
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