TU Darmstadt / ULB / TUprints

Characterization and Inkjet Printing of an RNA Aptamer for Paper-Based Biosensing of Ciprofloxacin

Jaeger, Jeannine ; Groher, Florian ; Stamm, Jacqueline ; Spiehl, Dieter ; Braun, Johannes ; Dörsam, Edgar ; Suess, Beatrix (2023)
Characterization and Inkjet Printing of an RNA Aptamer for Paper-Based Biosensing of Ciprofloxacin.
In: Biosensors, 2019, 9 (1)
doi: 10.26083/tuprints-00016878
Article, Secondary publication, Publisher's Version

Copyright Information: CC BY 4.0 International - Creative Commons, Attribution.

Download (21MB) | Preview
Item Type: Article
Type of entry: Secondary publication
Title: Characterization and Inkjet Printing of an RNA Aptamer for Paper-Based Biosensing of Ciprofloxacin
Language: English
Date: 20 November 2023
Place of Publication: Darmstadt
Year of primary publication: 2019
Place of primary publication: Basel
Publisher: MDPI
Journal or Publication Title: Biosensors
Volume of the journal: 9
Issue Number: 1
Collation: 10 Seiten
DOI: 10.26083/tuprints-00016878
Corresponding Links:
Origin: Secondary publication DeepGreen

The excessive use of antibiotics in food-producing animals causes a steady rise of multiple antibiotic resistance in foodborne bacteria. Next to sulfonamides, the most common antibiotics groups are fluoroquinolones, aminoglycosides, and ß-lactams. Therefore, there is a need for a quick, efficient, and low-cost detection procedure for antibiotics. In this study, we propose an inkjet-printed aptamer-based biosensor developed for the detection of the fluoroquinolone ciprofloxacin. Due to their extraordinary high affinity and specificity, aptamers are already widely used in various applications. Here we present a ciprofloxacin-binding RNA aptamer developed by systematic evolution of ligands by exponential enrichment (SELEX). We characterized the secondary structure of the aptamer and determined the KD to 36 nM that allow detection of antibiotic contamination in a relevant range. We demonstrate that RNA aptamers can be inkjet-printed, dried, and resolved while keeping their functionality consistently intact. With this proof of concept, we are paving the way for a potential range of additional aptamer-based, printable biosensors.

Uncontrolled Keywords: biosensor, aptamer, ciprofloxacin, detection, printing, fluorescence
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-168788
Additional Information:

This article belongs to the Special Issue Feature Papers: State-of-the-Art Biosensors Technology 2018

Classification DDC: 500 Science and mathematics > 570 Life sciences, biology
600 Technology, medicine, applied sciences > 620 Engineering and machine engineering
Divisions: 10 Department of Biology > Synthetic RNA biology
16 Department of Mechanical Engineering > Institute of Printing Science and Technology (IDD)
Date Deposited: 20 Nov 2023 15:07
Last Modified: 29 Nov 2023 09:12
SWORD Depositor: Deep Green
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/16878
PPN: 513536205
Actions (login required)
View Item View Item