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Toxic Effect of Vancomycin on Viability and Functionality of Different Cells Involved in Tissue Regeneration

Braun, Joy ; Eckes, Stefanie ; Rommens, Pol Maria ; Schmitz, Katja ; Nickel, Daniela ; Ritz, Ulrike (2023)
Toxic Effect of Vancomycin on Viability and Functionality of Different Cells Involved in Tissue Regeneration.
In: Antibiotics, 2020, 9 (5)
doi: 10.26083/tuprints-00016632
Article, Secondary publication, Publisher's Version

Copyright Information: CC BY 4.0 International - Creative Commons, Attribution.

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Item Type: Article
Type of entry: Secondary publication
Title: Toxic Effect of Vancomycin on Viability and Functionality of Different Cells Involved in Tissue Regeneration
Language: English
Date: 20 November 2023
Place of Publication: Darmstadt
Year of primary publication: 2020
Place of primary publication: Basel
Publisher: MDPI
Journal or Publication Title: Antibiotics
Volume of the journal: 9
Issue Number: 5
Collation: 15 Seiten
DOI: 10.26083/tuprints-00016632
Corresponding Links:
Origin: Secondary publication DeepGreen

To prevent infections local delivery of antibiotics is a useful tool. Especially in bone fractures, vancomycin impregnated bone cements are often used allowing high concentrations of antibiotics at the infection side without high serum concentrations. However, besides potential pathogens, cells involved in tissue regeneration may also be affected by the drug. We investigated the effect of vancomycin on the viability and functionality on osteoblasts, endothelial cells, fibroblasts and skeletal muscle cells. Our results show that the viability of all cells analyzed was reduced by vancomycin and that the observed effects were time and concentration dependent. The most pronounced toxic effect was detected on day three when even the lowest concentration of 0.01 mg/ml led to a significant decrease in proliferation compared to control. Functionality assays of osteoblasts and skeletal muscle cells revealed a sensitive reaction of the cells to the drug, indicating that vancomycin is toxic to these cells during the process of differentiation. These data suggest that the vancomycin administration is critical for cell survival and function. Therefore, the concentration of administered antibiotics needs to be carefully evaluated to find a balance between defense against pathogens and functionality of host cells and tissues.

Uncontrolled Keywords: vancomycin, cell proliferation, cell differentiation, tissue regeneration, local antibiotics
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-166329
Classification DDC: 500 Science and mathematics > 540 Chemistry
500 Science and mathematics > 570 Life sciences, biology
600 Technology, medicine, applied sciences > 610 Medicine and health
Divisions: 07 Department of Chemistry > Clemens-Schöpf-Institut > Fachgebiet Biochemie > Biologische Chemie
Date Deposited: 20 Nov 2023 14:52
Last Modified: 06 Dec 2023 09:27
SWORD Depositor: Deep Green
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/16632
PPN: 513689931
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