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Complete Genome Sequence of the Model Halovirus PhiH1 (ΦH1)

Dyall-Smith, Mike ; Pfeifer, Felicitas ; Witte, Angela ; Oesterhelt, Dieter ; Pfeiffer, Friedhelm (2023)
Complete Genome Sequence of the Model Halovirus PhiH1 (ΦH1).
In: Genes, 2018, 9 (10)
doi: 10.26083/tuprints-00016564
Article, Secondary publication, Publisher's Version

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Item Type: Article
Type of entry: Secondary publication
Title: Complete Genome Sequence of the Model Halovirus PhiH1 (ΦH1)
Language: English
Date: 21 November 2023
Place of Publication: Darmstadt
Year of primary publication: 2018
Place of primary publication: Basel
Publisher: MDPI
Journal or Publication Title: Genes
Volume of the journal: 9
Issue Number: 10
Collation: 20 Seiten
DOI: 10.26083/tuprints-00016564
Corresponding Links:
Origin: Secondary publication DeepGreen

The halophilic myohalovirus Halobacterium virus phiH (ΦH) was first described in 1982 and was isolated from a spontaneously lysed culture of Halobacterium salinarum strain R1. Until 1994, it was used extensively as a model to study the molecular genetics of haloarchaea, but only parts of the viral genome were sequenced during this period. Using Sanger sequencing combined with high-coverage Illumina sequencing, the full genome sequence of the major variant (phiH1) of this halovirus has been determined. The dsDNA genome is 58,072 bp in length and carries 97 protein-coding genes. We have integrated this information with the previously described transcription mapping data. PhiH could be classified into Myoviridae Type1, Cluster 4 based on capsid assembly and structural proteins (VIRFAM). The closest relative was Natrialba virus phiCh1 (φCh1), which shared 63% nucleotide identity and displayed a high level of gene synteny. This close relationship was supported by phylogenetic tree reconstructions. The complete sequence of this historically important virus will allow its inclusion in studies of comparative genomics and virus diversity.

Uncontrolled Keywords: halovirus, virus, halophage, Halobacterium salinarum, Archaea, haloarchaea, halobacteria, genome inversion
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-165647
Additional Information:

This article belongs to the Special Issue Genetics of Halophilic Microorganisms

Classification DDC: 500 Science and mathematics > 570 Life sciences, biology
Divisions: 10 Department of Biology > Microbiology and Archaea
Date Deposited: 21 Nov 2023 13:34
Last Modified: 09 Jan 2024 08:59
SWORD Depositor: Deep Green
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/16564
PPN: 514512733
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