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Bioprinting Cell- and Spheroid-Laden Protein-Engineered Hydrogels as Tissue-on-Chip Platforms

Duarte Campos, Daniela F. ; Lindsay, Christopher D. ; Roth, Julien G. ; LeSavage, Bauer L. ; Seymour, Alexis J. ; Krajina, Brad A. ; Ribeiro, Ricardo ; Costa, Pedro F. ; Blaeser, Andreas ; Heilshorn, Sarah C. (2023)
Bioprinting Cell- and Spheroid-Laden Protein-Engineered Hydrogels as Tissue-on-Chip Platforms.
In: Frontiers in Bioengineering and Biotechnology, 2020, 8
doi: 10.26083/tuprints-00016294
Article, Secondary publication, Publisher's Version

Copyright Information: CC BY 4.0 International - Creative Commons, Attribution.

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Item Type: Article
Type of entry: Secondary publication
Title: Bioprinting Cell- and Spheroid-Laden Protein-Engineered Hydrogels as Tissue-on-Chip Platforms
Language: English
Date: 8 December 2023
Place of Publication: Darmstadt
Year of primary publication: 2020
Place of primary publication: Lausanne
Publisher: Frontiers Media S.A.
Journal or Publication Title: Frontiers in Bioengineering and Biotechnology
Volume of the journal: 8
Collation: 13 Seiten
DOI: 10.26083/tuprints-00016294
Corresponding Links:
Origin: Secondary publication DeepGreen

Human tissues, both in health and disease, are exquisitely organized into complex three-dimensional architectures that inform tissue function. In biomedical research, specifically in drug discovery and personalized medicine, novel human-based three-dimensional (3D) models are needed to provide information with higher predictive value compared to state-of-the-art two-dimensional (2D) preclinical models. However, current in vitro models remain inadequate to recapitulate the complex and heterogenous architectures that underlie biology. Therefore, it would be beneficial to develop novel models that could capture both the 3D heterogeneity of tissue (e.g., through 3D bioprinting) and integrate vascularization that is necessary for tissue viability (e.g., through culture in tissue-on-chips). In this proof-of-concept study, we use elastin-like protein (ELP) engineered hydrogels as bioinks for constructing such tissue models, which can be directly dispensed onto endothelialized on-chip platforms. We show that this bioprinting process is compatible with both single cell suspensions of neural progenitor cells (NPCs) and spheroid aggregates of breast cancer cells. After bioprinting, both cell types remain viable in incubation for up to 14 days. These results demonstrate a first step toward combining ELP engineered hydrogels with 3D bioprinting technologies and on-chip platforms comprising vascular-like channels for establishing functional tissue models.

Uncontrolled Keywords: protein engineered hydrogel, bioink, bioprinting, 3D cell culture, tissue model
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-162945
Additional Information:

This article is part of the Research Topic: 3D Printing for Implantable Medical Devices: From Surgical Reconstruction to Tissue/Organ Regeneration

Classification DDC: 500 Science and mathematics > 570 Life sciences, biology
600 Technology, medicine, applied sciences > 610 Medicine and health
600 Technology, medicine, applied sciences > 620 Engineering and machine engineering
Divisions: 16 Department of Mechanical Engineering > Institute of Printing Science and Technology (IDD) > Biomedical Printing Technology (BMT)
Date Deposited: 08 Dec 2023 14:51
Last Modified: 15 Dec 2023 10:36
SWORD Depositor: Deep Green
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/16294
PPN: 513962867
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