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The Effect of X-Ray and Heavy Ions Radiations on Chemotherapy Refractory Tumor Cells

Yu, Zhan ; Hartel, Carola ; Pignalosa, Diana ; Kraft-Weyrather, Wilma ; Jiang, Guo-Liang ; Diaz-Carballo, David ; Durante, Marco (2024)
The Effect of X-Ray and Heavy Ions Radiations on Chemotherapy Refractory Tumor Cells.
In: Frontiers in Oncology, 2016, 6
doi: 10.26083/tuprints-00016228
Article, Secondary publication, Publisher's Version

Copyright Information: CC BY 4.0 International - Creative Commons, Attribution.

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Item Type: Article
Type of entry: Secondary publication
Title: The Effect of X-Ray and Heavy Ions Radiations on Chemotherapy Refractory Tumor Cells
Language: English
Date: 8 March 2024
Place of Publication: Darmstadt
Year of primary publication: 29 March 2016
Place of primary publication: Lausanne
Publisher: Frontiers Media S.A.
Journal or Publication Title: Frontiers in Oncology
Volume of the journal: 6
Collation: 9 Seiten
DOI: 10.26083/tuprints-00016228
Corresponding Links:
Origin: Secondary publication DeepGreen

Purpose: The purpose of this study is to link both numeric and structural chromosomal aberrations to the effectiveness of radiotherapy in chemotherapy refractory tumor cells.

Materials and methods: Neuroblastoma (LAN-1) and 79HF6 glioblastoma cells derived from patients and their chemoresistant sublines were artificially cultured as neurospheres and irradiated by X-rays and heavy ions sources. All the cell lines were irradiated by Carbon-SIS with LET of 100 keV/μm. However, 79HF6 cells and LAN-1 cells were also irradiated by Carbon-UNILAC with LET of 168 keV/μm and Nickel ions with LET of 174 keV/μm, respectively. The effect of radiation on the survival and proliferation of cells was addressed by standard clonogenic assays. In order to analyze cell karyotype standard Giemsa staining, multicolor fluorescence in situ hybridization (mFISH) and multicolor banding (mBAND) techniques were applied.

Results: Relative biological effectiveness values of heavy ion beams relative to X-rays at the D10 values were found between 2.3 and 2.6 with Carbon-SIS and Nickel for LAN-1 and between 2.5 and 3.4 with Carbon-SIS and Carbon-UNILAC for 79HF6 cells. Chemorefractory LAN-1RETO cells were found more radioresistant than untreated LAN-1WT cells. 79HF6RETO glioblastoma cells were found more radiosensitive than cytostatic sensitive cells 79HF6WT. Sphere formation assay showed that LAN-1RETO cells were able to form spheres in serum-free culture, whereas 79HF6 cells could not. Most of 79HF6WT cells revealed a number of 71–90 chromosomes, whereas 79HF6RETO revealed a number of 52–83 chromosomes. The majority of LAN-1WT cells revealed a number of 40–44 chromosomes. mFISH analysis showed some stable aberrations, especially on chromosome 10 as judged by the impossibility to label this region with specific probes. This was corroborated using mBAND analysis.

Conclusion: Heavy ion irradiation was more effective than X-ray in both cytostatic naive cancer and chemoresistant cell lines. LAN-1RETO chemoresistant neuroblastoma cells were found to be more radioresistant than the cytostatic naive cells (LAN-1WT), whereas this effect was not found in 79HF6 cells.

Uncontrolled Keywords: chemoresistance, X-ray and heavy ion irradiation, relative biological effectiveness, neuroblastoma, glioblastoma
Identification Number: Artikel-ID: 64
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-162283
Additional Information:

This article is part of the Research Topic: Charged Particles in Oncology

Specialty section: This article was submitted to Radiation Oncology, a section of the journal Frontiers in Oncology

Classification DDC: 500 Science and mathematics > 530 Physics
600 Technology, medicine, applied sciences > 610 Medicine and health
Divisions: 05 Department of Physics > Institute for Condensed Matter Physics
Date Deposited: 08 Mar 2024 13:04
Last Modified: 30 Apr 2024 12:32
SWORD Depositor: Deep Green
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/16228
PPN: 517678187
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