TU Darmstadt / ULB / TUprints

Challenges and Perspectives in Chemical Synthesis of Highly Hydrophobic Peptides

Mueller, Lena K. ; Baumruck, Andreas C. ; Zhdanova, Hanna ; Tietze, Alesia A. (2024)
Challenges and Perspectives in Chemical Synthesis of Highly Hydrophobic Peptides.
In: Frontiers in Bioengineering and Biotechnology, 2020, 8
doi: 10.26083/tuprints-00016102
Article, Secondary publication, Publisher's Version

Copyright Information: CC BY 4.0 International - Creative Commons, Attribution.

Download (5MB) | Preview
Item Type: Article
Type of entry: Secondary publication
Title: Challenges and Perspectives in Chemical Synthesis of Highly Hydrophobic Peptides
Language: English
Date: 8 March 2024
Place of Publication: Darmstadt
Year of primary publication: 4 March 2020
Place of primary publication: Lausanne
Publisher: Frontiers Media S.A.
Journal or Publication Title: Frontiers in Bioengineering and Biotechnology
Volume of the journal: 8
Collation: 17 Seiten
DOI: 10.26083/tuprints-00016102
Corresponding Links:
Origin: Secondary publication DeepGreen

Solid phase peptide synthesis (SPPS) provides the possibility to chemically synthesize peptides and proteins. Applying the method on hydrophilic structures is usually without major drawbacks but faces extreme complications when it comes to "difficult sequences." These includes the vitally important, ubiquitously present and structurally demanding membrane proteins and their functional parts, such as ion channels, G-protein receptors, and other pore-forming structures. Standard synthetic and ligation protocols are not enough for a successful synthesis of these challenging sequences. In this review we highlight, summarize and evaluate the possibilities for synthetic production of "difficult sequences" by SPPS, native chemical ligation (NCL) and follow-up protocols.

Uncontrolled Keywords: solid phase peptide synthesis, membrane-associated proteins, native chemical ligation, conjugation, transmembrane peptide
Identification Number: Artikel-ID: 162
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-161026
Additional Information:

This article is part of the Research Topic: Engineering of Biological Membranes

Specialty section: This article was submitted to Synthetic Biology, a section of the journal Frontiers in Bioengineering and Biotechnology

Classification DDC: 500 Science and mathematics > 540 Chemistry
500 Science and mathematics > 570 Life sciences, biology
Divisions: 07 Department of Chemistry > Clemens-Schöpf-Institut > Fachgebiet Biochemie
Date Deposited: 08 Mar 2024 13:24
Last Modified: 11 Jun 2024 12:19
SWORD Depositor: Deep Green
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/16102
PPN: 519032527
Actions (login required)
View Item View Item