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Nanofluidic Immobilization and Growth Detection of Escherichia coli in a Chip for Antibiotic Susceptibility Testing

Busche, Jan F. ; Möller, Svenja ; Klein, Ann-Kathrin ; Stehr, Matthias ; Purr, Foelke ; Bassu, Margherita ; Burg, Thomas P. ; Dietzel, Andreas (2022):
Nanofluidic Immobilization and Growth Detection of Escherichia coli in a Chip for Antibiotic Susceptibility Testing. (Publisher's Version)
In: Biosensors, 10 (10), MDPI, e-ISSN 2079-6374,
DOI: 10.26083/tuprints-00015962,
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Item Type: Article
Origin: Secondary publication DeepGreen
Status: Publisher's Version
Title: Nanofluidic Immobilization and Growth Detection of Escherichia coli in a Chip for Antibiotic Susceptibility Testing
Language: English
Abstract:

Infections with antimicrobial resistant bacteria are a rising threat for global healthcare as more and more antibiotics lose their effectiveness against bacterial pathogens. To guarantee the long-term effectiveness of broad-spectrum antibiotics, they may only be prescribed when inevitably required. In order to make a reliable assessment of which antibiotics are effective, rapid point-of-care tests are needed. This can be achieved with fast phenotypic microfluidic tests, which can cope with low bacterial concentrations and work label-free. Here, we present a novel optofluidic chip with a cross-flow immobilization principle using a regular array of nanogaps to concentrate bacteria and detect their growth label-free under the influence of antibiotics. The interferometric measuring principle enabled the detection of the growth of Escherichia coli in under 4 h with a sample volume of 187.2 µL and a doubling time of 79 min. In proof-of-concept experiments, we could show that the method can distinguish between bacterial growth and its inhibition by antibiotics. The results indicate that the nanofluidic chip approach provides a very promising concept for future rapid and label-free antimicrobial susceptibility tests.

Journal or Publication Title: Biosensors
Volume of the journal: 10
Issue Number: 10
Publisher: MDPI
Collation: 12 Seiten
Uncontrolled Keywords: optofluidic, nanofluidic, antibiotic resistance test, nano-grating, microfabrication
Classification DDC: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie
600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften
Divisions: 18 Department of Electrical Engineering and Information Technology > Integrated Micro- and Nanosystems
Date Deposited: 09 Feb 2022 14:56
Last Modified: 02 May 2022 12:07
DOI: 10.26083/tuprints-00015962
Corresponding Links:
URN: urn:nbn:de:tuda-tuprints-159624
SWORD Depositor: Deep Green
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/15962
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