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A Microfluidic Split-Flow Technology for Product Characterization in Continuous Low-Volume Nanoparticle Synthesis

Bolze, Holger ; Erfle, Peer ; Riewe, Juliane ; Bunjes, Heike ; Dietzel, Andreas ; Burg, Thomas P. (2023)
A Microfluidic Split-Flow Technology for Product Characterization in Continuous Low-Volume Nanoparticle Synthesis.
In: Micromachines, 2019, 10 (3)
doi: 10.26083/tuprints-00015928
Article, Secondary publication, Publisher's Version

Copyright Information: CC BY 4.0 International - Creative Commons, Attribution.

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Item Type: Article
Type of entry: Secondary publication
Title: A Microfluidic Split-Flow Technology for Product Characterization in Continuous Low-Volume Nanoparticle Synthesis
Language: English
Date: 4 December 2023
Place of Publication: Darmstadt
Year of primary publication: 2019
Place of primary publication: Basel
Publisher: MDPI
Journal or Publication Title: Micromachines
Volume of the journal: 10
Issue Number: 3
Collation: 16 Seiten
DOI: 10.26083/tuprints-00015928
Corresponding Links:
Origin: Secondary publication DeepGreen

A key aspect of microfluidic processes is their ability to perform chemical reactions in small volumes under continuous flow. However, a continuous process requires stable reagent flow over a prolonged period. This can be challenging in microfluidic systems, as bubbles or particles easily block or alter the flow. Online analysis of the product stream can alleviate this problem by providing a feedback signal. When this signal exceeds a pre-defined range, the process can be re-adjusted or interrupted to prevent contamination. Here we demonstrate the feasibility of this concept by implementing a microfluidic detector downstream of a segmented-flow system for the synthesis of lipid nanoparticles. To match the flow rate through the detector to the measurement bandwidth independent of the synthesis requirements, a small stream is sidelined from the original product stream and routed through a measuring channel with 2 × 2 µm cross-section. The small size of the measuring channel prevents the entry of air plugs, which are inherent to our segmented flow synthesis device. Nanoparticles passing through the small channel were detected and characterized by quantitative fluorescence measurements. With this setup, we were able to count single nanoparticles. This way, we were able to detect changes in the particle synthesis affecting the size, concentration, or velocity of the particles in suspension. We envision that the flow-splitting scheme demonstrated here can be transferred to detection methods other than fluorescence for continuous monitoring and feedback control of microfluidic nanoparticle synthesis.

Uncontrolled Keywords: lipid nanoparticles, online analysis, microfluidics, plug flow mixer, fluorescence, precipitation, single particle analysis, nanoparticle characterization
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-159280
Additional Information:

This article belongs to the Special Issue Micro- and Nanofluidics for Bionanoparticle Analysis

Classification DDC: 600 Technology, medicine, applied sciences > 621.3 Electrical engineering, electronics
Divisions: 18 Department of Electrical Engineering and Information Technology > Integrated Micro- and Nanosystems
Date Deposited: 04 Dec 2023 10:20
Last Modified: 13 Dec 2023 13:43
SWORD Depositor: Deep Green
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/15928
PPN: 513918167
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