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Robust Smartphone Assisted Biosensing Based on Asymmetric Nanofluidic Grating Interferometry

Purr, Foelke ; Eckardt, Max-Frederik ; Kieserling, Jonas ; Gronwald, Paul-Luis ; Burg, Thomas P. ; Dietzel, Andreas (2023)
Robust Smartphone Assisted Biosensing Based on Asymmetric Nanofluidic Grating Interferometry.
In: Sensors, 2019, 19 (9)
doi: 10.26083/tuprints-00015864
Article, Secondary publication, Publisher's Version

Copyright Information: CC BY 4.0 International - Creative Commons, Attribution.

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Item Type: Article
Type of entry: Secondary publication
Title: Robust Smartphone Assisted Biosensing Based on Asymmetric Nanofluidic Grating Interferometry
Language: English
Date: 4 December 2023
Place of Publication: Darmstadt
Year of primary publication: 2019
Place of primary publication: Basel
Publisher: MDPI
Journal or Publication Title: Sensors
Volume of the journal: 19
Issue Number: 9
Collation: 14 Seiten
DOI: 10.26083/tuprints-00015864
Corresponding Links:
Origin: Secondary publication DeepGreen

Point-of-care systems enable fast therapy decisions on site without the need of any healthcare infrastructure. In addition to the sensitive detection, stable measurement by inexperienced persons outside of laboratory facilities is indispensable. A particular challenge in field applications is to reduce interference from environmental factors, such as temperature, to acceptable levels without sacrificing simplicity. Here, we present a smartphone-based point-of-care sensor. The method uses an optofluidic grating composed of alternating detection and reference channels arranged as a reflective phase grating. Biomolecules adsorbing to the detection channel alter the optical path length, while the parallel reference channels enable a direct common mode rejection within a single measurement. The optical setup is integrated in a compact design of a mobile readout device and the usability is ensured by a smartphone application. Our results show that different ambient temperatures do not have any influence on the signal. In a proof-of concept experiment we measured the accumulation of specific molecules in functionalized detection channels in real-time and without the need of any labeling. Therefore, the channel walls have been modified with biotin as capture molecules and the specific binding of streptavidin was detected. A mobile, reliable and robust point-of-care device has been realized by combining an inherently differential measurement concept with a smartphone-based, mobile readout device.

Uncontrolled Keywords: biosensing, interferometry, portable point-of-care (POC), common mode rejection, nanofluidic, optofluidic grating, smartphone-based
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-158648
Additional Information:

This article belongs to the Special Issue Portable Biosensing Systems for Point-of-Care Diagnostic Applications

Classification DDC: 600 Technology, medicine, applied sciences > 621.3 Electrical engineering, electronics
Divisions: 18 Department of Electrical Engineering and Information Technology > Integrated Micro- and Nanosystems
Date Deposited: 04 Dec 2023 10:21
Last Modified: 13 Dec 2023 13:44
SWORD Depositor: Deep Green
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/15864
PPN: 513918000
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