TU Darmstadt / ULB / TUprints

Phosphoprotein Detection with a Single Nanofluidic Diode Decorated with Zinc Chelates

Nasir, Saima ; Ali, Mubarak ; Ahmed, Ishtiaq ; Niemeyer, Christof M. ; Ensinger, Wolfgang (2020)
Phosphoprotein Detection with a Single Nanofluidic Diode Decorated with Zinc Chelates.
In: ChemPlusChem, 2020, 85 (3)
doi: 10.25534/tuprints-00011604
Article, Secondary publication

Copyright Information: CC BY 4.0 International - Creative Commons, Attribution.

Download (5MB) | Preview
Item Type: Article
Type of entry: Secondary publication
Title: Phosphoprotein Detection with a Single Nanofluidic Diode Decorated with Zinc Chelates
Language: English
Date: 31 March 2020
Place of Publication: Darmstadt
Year of primary publication: 2020
Publisher: Wiley-VCH
Journal or Publication Title: ChemPlusChem
Volume of the journal: 85
Issue Number: 3
DOI: 10.25534/tuprints-00011604
URL / URN: https://doi.org/10.1002/cplu.202000045
Origin: Secondary publication via DEAL-contract with Wiley

We report a nanofluidic device for the label-free detection of phosphoprotein (PPn) analytes. To achieve this goal, a metal ion chelator, namely 4-[bis(2-pyridylmethyl)aminomethyl]aniline (DPA-NH2) compound was synthesized. Single asymmetric nanofluidic channels were fabricated in polyethylene terephthalate (PET) membranes. The chelator (DPA-NH2) molecules are subsequently immobilized on the nanochannel surface, followed by the zinc ion complexation to afford DPA-Zn2+ chelates, which act as ligand moieties for the specific binding of phosphoproteins. The success of the chemical reaction and biomolecular recognition process that occur in a confined geometry can be monitored from the changes in electrical readout of the nanochannel. The nanofluidic sensor has the ability to sensitively and specifically detect lower concentrations (≥1 nM) of phosphoprotein (albumin and α-casein) in the surrounding environment as evidenced from the significant decrease in ion current flowing through the nanochannels. However, dephosphoproteins such as lysozyme and dephospho- α-casein even at higher concentration (>1 μM) could not induce any significant change in the transmembrane ion flux. This observation indicated the sensitivity and specificity of the proposed nanofluidic sensor towards PPn proteins, and has potential for use in differentiating between phosphoproteins and dephosphoproteins.

URN: urn:nbn:de:tuda-tuprints-116041
Classification DDC: 600 Technology, medicine, applied sciences > 600 Technology
Divisions: 11 Department of Materials and Earth Sciences > Material Science > Material Analytics
Date Deposited: 31 Mar 2020 11:44
Last Modified: 14 Nov 2023 19:03
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/11604
Actions (login required)
View Item View Item