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3D-printable portable open-source platform for low-cost lens-less holographic cellular imaging

Amann, Stephan and Witzleben, Max von and Breuer, Stefan (2019):
3D-printable portable open-source platform for low-cost lens-less holographic cellular imaging.
9, In: Scientific Reports, (1), Springer Nature, ISSN 2045-2322, DOI: 10.1038/s41598-019-47689-1,
Secondary publishing via sponsored Golden Open Access, [Article]

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Item Type: Article
Origin: Secondary publishing via sponsored Golden Open Access
Title: 3D-printable portable open-source platform for low-cost lens-less holographic cellular imaging
Language: English
Abstract:

Digital holographic microscopy is an emerging, potentially low-cost alternative to conventional light microscopy for micro-object imaging on earth, underwater and in space. Immediate access to micronscale objects however requires a well-balanced system design and sophisticated reconstruction algorithms, that are commercially available, however not accessible cost-efficiently. Here, we present an open-source implementation of a lens-less digital inline holographic microscope platform, based on off-the-shelf optical, electronic and mechanical components, costing less than $190. It employs a Blu- Ray semiconductor-laser-pickup or a light-emitting-diode, a pinhole, a 3D-printed housing consisting of 3 parts and a single-board portable computer and camera with an open-source implementation of the Fresnel-Kirchhoff routine. We demonstrate 1.55 μm spatial resolution by laser-pickup and 3.91 μm by the light-emitting-diode source. The housing and mechanical components are 3D printed. Both printer and reconstruction software source codes are open. The light-weight microscope allows to image label-free micro-spheres of 6.5 μm diameter, human red-blood-cells of about 8 μm diameter as well as fast-growing plant Nicotiana-tabacum-BY-2 suspension cells with 50 μm sizes. The imaging capability is validated by imaging-contrast quantification involving a standardized test target. The presented 3D-printable portable open-source platform represents a fully-open design, low-cost modular and versatile imaging-solution for use in high- and low-resource areas of the world.

Journal or Publication Title: Scientific Reports
Volume: 9
Number: 1
Publisher: Springer Nature
Classification DDC: 500 Naturwissenschaften und Mathematik > 530 Physik
Divisions: 05 Department of Physics > Institute of Applied Physics > Semiconductor Optics Group
Date Deposited: 11 Sep 2019 12:40
Last Modified: 21 Jan 2020 15:06
DOI: 10.1038/s41598-019-47689-1
URN: urn:nbn:de:tuda-tuprints-90817
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/9081
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