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Lipid-rafts remain stable even after ionizing radiation induced disintegration of β1 integrin containing focal adhesions

Babel, Laura and Kruse, Larissa and Bump, Steven and Langhans, Markus and Meckel, Tobias :
Lipid-rafts remain stable even after ionizing radiation induced disintegration of β1 integrin containing focal adhesions.
[Online-Edition]
In: BMC Research Notes, 10 (1) ISSN 1756-0500
[Article], (2017)

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Item Type: Article
Title: Lipid-rafts remain stable even after ionizing radiation induced disintegration of β1 integrin containing focal adhesions
Language: English
Abstract:

Objective: Adhesion of cells to the extracellular matrix is facilitated by integrin receptors. We recently found that a nanoscale organization of plasma membrane located integrins containing the β1 subunit is responsible for an enhanced radio-resistance in 3D cultured cells over cells grown in 2D. While ionizing radiation is known to have broad effects on the lipid composition of the plasma membrane and their organization in lipid-rafts, it is not clear whether the effects of ionizing radiation on the nanoscale clustering of integrins is lipid-raft dependent. Results: Using single molecule microscopy we can show that β1 integrins colocalize with cholesterol in lipid-rafts. Ionizing radiation, as an extrinsic stressor, causes the separation of β1 integrins from cholesterol lipid raft suggesting that the effects of ionizing radiation on the clustering of β1 integrins are lipid-raft independent.

Journal or Publication Title: BMC Research Notes
Volume: 10
Number: 1
Classification DDC: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie
Divisions: 10 Department of Biology > Membrane Dynamics
Date Deposited: 14 Dec 2017 14:10
Last Modified: 14 Dec 2017 14:10
Official URL: https://doi.org/10.1186/s13104-017-3032-8
URN: urn:nbn:de:tuda-tuprints-70428
URI: http://tuprints.ulb.tu-darmstadt.de/id/eprint/7042
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