• Supplements
• Related work
• Publisher-DOI

3D molecular structure determination is a challenge for organic compounds or natural products available in minute amounts. Proton/proton and proton/carbon correlations yield the constitution. J couplings and NOEs oftentimes supported by one-bond ¹H,¹³C residual dipolar couplings (RDCs) or by ¹³C residual chemical shift anisotropies (RCSAs) provide the relative configuration. However, these RDCs or carbon RCSAs rely on 1% natural abundance of ¹³C preventing their use for compounds available only in quantities of a few 10’s of µgs. By contrast, ¹H RCSAs provide similar information on spatial orientation of structural moieties within a molecule, while using the abundant ¹H spin. Herein, ¹H RCSAs are accurately measured using constrained aligning gels or liquid crystals and applied to the 3D structural determination of molecules with varying complexities. Even more, deuterated alignment media allow the elucidation of the relative configuration of around 35 µg of a briarane compound isolated from Briareum asbestinum.

"The original version of the Supplementary Information associated with this Article contained errors in Supplementary Fig. 9a and on page 7. In both cases, chemical formula of the liquid crystalline medium was drawn incorrectly. The HTML has been updated to include a corrected version of the Supplementary Information; the original incorrect versions of these Figs. can be found as Supplementary Information associated with this Correction."