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A single-cylinder full-metal engine with a real combustion chamber geometry was used to investigate particulate number emissions resulting from transient engine operation. The formation of particulate number emissions depends on mixture formation influenced by the in-cylinder flow and injection, and the formation of fuel films on the in-cylinder walls. For the investigation of this multi-parameter process, simultaneous endoscopic PIV and combustion visualization were applied. Hence, the measurement techniques allowed the investigation of in-cylinder flow, flame propagation, and soot formation. The test rig was modified to apply a generic load step and a realistic tip-in with Miller cycle. The reproducibility of the engine parameters during the transient allowed statistical analysis and the comparison between steady-state operating points. Cause-and-effect chains concerning the formation of soot are concluded by correlation analysis of parameters extracted from the flow field, the flame propagation and the soot luminosity.