On the 4th and 5th of November 2021, more than 50 scholars from different disciplines and countries came together in an online conference to discuss the multiple aspects of Transformations of Infrastructure Systems at the second international conference organized by the Research Training Group KRITIS at the Technical University of Darmstadt, Germany. The focus of this conference was on the dynamic and changing nature of infrastructure systems and describing, understanding, and explaining transformation processes of infrastructures. Within the four multidisciplinary panels (Safety, Cultures, Governance, and both Temporality and Spatiality) the participants shared their research and knowledge on various aspects of transformation of infrastructure Systems. The conference gave an insight into the triggers of transformations and highlighted the conditions under which they take place and the consequences. The keynote lectures by Prof. Dr. Timothy Moss (Humboldt University of Berlin), Dr. Anique Hommels (Maastricht University), and Niklas Vespermann (Federal Network Agency, Germany) further highlighted and deepened the aspects relevant to this context.