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Enzyme-Polymer Conjugates to Enhance Enzyme Shelf Life in a Liquid Detergent Formulation

Kübelbeck, Sonja ; Mikhael, Jules ; Keller, Harald ; Konradi, Rupert ; Andrieu-Brunsen, Annette ; Baier, Grit (2022)
Enzyme-Polymer Conjugates to Enhance Enzyme Shelf Life in a Liquid Detergent Formulation.
In: Macromolecular Bioscience, 2018, 18 (7)
doi: 10.26083/tuprints-00021680
Article, Secondary publication, Postprint

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Item Type: Article
Type of entry: Secondary publication
Title: Enzyme-Polymer Conjugates to Enhance Enzyme Shelf Life in a Liquid Detergent Formulation
Language: English
Date: 2022
Place of Publication: Darmstadt
Year of primary publication: 2018
Publisher: John Wiley & Sons
Journal or Publication Title: Macromolecular Bioscience
Volume of the journal: 18
Issue Number: 7
Collation: 26 Seiten
DOI: 10.26083/tuprints-00021680
Corresponding Links:
Origin: Secondary publication service
Abstract:

Herein, the synthesis of enzyme–polymer conjugates is reported. Four different activated polymers (mPEG-aldehyde, mPEG-NHS, maltodextrin-aldehyde, carboxymethyl cellulose aldehyde) are conjugated to the surface of protease, α-amylase, and lipase using two different strategies (reductive amination and alkylation with NHS-activated acid). Although the chemical modification of the enzymes is accompanied by losses in enzyme activity (maximum loss 40%), the covalent attachment of polymers increases the thermal stability and the stability in a standard detergent formulation compared to the unmodified enzymes. The enzyme–polymer conjugates are characterized by asymmetrical-flow field-flow fractionation and differential scanning microcalorimetry. Furthermore, it is demonstrated that conjugated enzymes still show performance in a real washing process. Enzyme–polymer conjugates show a potential as a stabilizing system for enzymes in detergents.

Status: Postprint
URN: urn:nbn:de:tuda-tuprints-216808
Classification DDC: 500 Science and mathematics > 540 Chemistry
Divisions: Profile Areas > Thermo-Fluids & Interfaces
07 Department of Chemistry > Ernst-Berl-Institut > Fachgebiet Makromolekulare Chemie
Date Deposited: 20 Jul 2022 12:17
Last Modified: 10 Jan 2023 09:20
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/21680
PPN: 503428027
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