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Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles

Gawlitza, Kornelia ; Wu, Changzhu ; Georgieva, Radostina ; Ansorge-Schumacher, Marion ; Klitzing, Regine von (2022)
Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles.
In: Zeitschrift für Physikalische Chemie, 2012, 226 (7-8)
doi: 10.26083/tuprints-00019949
Article, Secondary publication, Publisher's Version

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Item Type: Article
Type of entry: Secondary publication
Title: Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles
Language: English
Date: 2022
Place of Publication: Darmstadt
Year of primary publication: 2012
Publisher: De Gruyter
Journal or Publication Title: Zeitschrift für Physikalische Chemie
Volume of the journal: 226
Issue Number: 7-8
DOI: 10.26083/tuprints-00019949
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Origin: Secondary publication service
Abstract:

The immobilization of lipase B from Candida antarctica(CalB) within micronsized poly-N-Isopropylacrylamide (p-NIPAM) microgel particles with a crosslinker content of 5% is reported. The immobilization of the enzyme was reached by an exchange from polar to organic solvents. After determining the embedded amount of CalB within the polymer network, an enhanced specificactivity inn-hexane was obtained. Due to the thermoresponsibility of the polymer particles, the activity reaction was done at 25°C and 50°C. The results presented show that the reversiblecollapse of the microgel leads to a decreased activity with increasing temperature. Hence, p-NIPAMmicrogels display a good opportunity to tailor the activity of CalB. An interesting side effect is that CalB presents a suitable probe to estimate the mesh size of the polymer network, since itpenetrates in the unlabeled form but not after labeling with FITC.

Uncontrolled Keywords: Lipase B; p-NIPAM; Immobilization; Catalytic Activity; Thermoresponsibility
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-199499
Classification DDC: 500 Science and mathematics > 530 Physics
500 Science and mathematics > 540 Chemistry
Divisions: 05 Department of Physics > Institute for Condensed Matter Physics
Date Deposited: 06 Dec 2022 12:27
Last Modified: 20 Mar 2023 08:15
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/19949
PPN: 506130223
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