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Covalent Attachment of Enzymes to Paper Fibers for Paper-Based Analytical Devices

Böhm, Alexander ; Trosien, Simon ; Avrutina, Olga ; Kolmar, Harald ; Biesalski, Markus (2024)
Covalent Attachment of Enzymes to Paper Fibers for Paper-Based Analytical Devices.
In: Frontiers in Chemistry, 2018, 6
doi: 10.26083/tuprints-00015860
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Item Type: Article
Type of entry: Secondary publication
Title: Covalent Attachment of Enzymes to Paper Fibers for Paper-Based Analytical Devices
Language: English
Date: 5 March 2024
Place of Publication: Darmstadt
Year of primary publication: 27 June 2018
Place of primary publication: Lausanne
Publisher: Frontiers Media S.A.
Journal or Publication Title: Frontiers in Chemistry
Volume of the journal: 6
Collation: 10 Seiten
DOI: 10.26083/tuprints-00015860
Corresponding Links:
Origin: Secondary publication DeepGreen
Abstract:

Due to its unique material properties, paper offers many practical advantages as a viable platform for sensing devices. In view of paper-based microfluidic biosensing applications, the covalent immobilization of enzymes with preserved functional activity is highly desirable and ultimately challenging. In the present manuscript, we report an efficient approach to achieving the covalent attachment of certain enzymes on paper fibers via a surface-bound network of hydrophilic polymers bearing protein-modifiable sites. This tailor-made macromolecular system consisting of polar, highly swellable copolymers is anchored to the paper exterior upon light-induced crosslinking of engineered benzophenone motifs. On the other hand, this framework contains active esters that can be efficiently modified by the nucleophiles of biomolecules. This strategy allowed the covalent immobilization of glucose oxidase and horseradish peroxidase onto cotton linters without sacrificing their bioactivities and performance upon surface binding. As a proof-of-concept application, a microfluidic chromatic paper-based glucose sensor was developed and achieved successful glucose detection in a simple yet efficient cascade reaction.

Uncontrolled Keywords: enzyme immobilization, biofunctional paper, lab-on-paper, microfluidics, paper-based diagnostics, point-of-care diagnostics, glucose sensor
Identification Number: Artikel-ID: 214
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-158603
Additional Information:

This article is part of the Research Topic: Sensing MY Biosphere

Specialty section: This article was submitted to Chemical Engineering, a section of the journal Frontiers in Chemistry

Classification DDC: 500 Science and mathematics > 540 Chemistry
Divisions: 07 Department of Chemistry > Clemens-Schöpf-Institut > Fachgebiet Biochemie
07 Department of Chemistry > Ernst-Berl-Institut > Fachgebiet Makromolekulare Chemie > Macromolecular and paper chemistry
Date Deposited: 05 Mar 2024 13:43
Last Modified: 24 Jun 2024 13:30
SWORD Depositor: Deep Green
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/15860
PPN: 519350987
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