Entwicklung Fluoreszenz-basierter Assays zur kinetischen Untersuchung neuer Enzymvarianten

Entwicklung verschiedener photometrischer und Fluoreszenz-basierter Assays zur untersuchung künstlich erzeugter Varianten der Fruktose-6-phosphat Aldolase, sowie Synthese der dafür benötigten Substrate und Erzeugung dere Enzym-Varianten mittels Punktmutation.

Entwicklung einer modularen Synthese für strukturverwandte enantiomeren reiner Aminsubstrate und Entwicklung des ersten Fluoreszenz-Basierten Assays zu kinetischen Untersuchung von w-Transaminasen

Development of several photometric and fluorescence-based assays for the screening of artificial variants of the Fructose-6-phosphate aldolase towards non-natural reactions. The project included the establishment of a modular synthesis for the substrates as well as planning point mutations to improve the enzyme’s activity and developing new assay concepts.

Development of a modular synthesis of structurally related enantio-pure chirale amines from corresponding alcohols created by Grignard-addition. The amines, bearing a methoxinaphthyl-based fluorophore, were later to be used as assay substrates for screening of w-transaminases.