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¹H, ¹³C and ¹⁵N assignment of stem-loop SL1 from the 5'-UTR of SARS-CoV-2

Richter, Christian ; Hohmann, Katharina F. ; Toews, Sabrina ; Mathieu, Daniel ; Altincekic, Nadide ; Bains, Jasleen Kaur ; Binas, Oliver ; Ceylan, Betül ; Duchardt-Ferner, Elke ; Ferner, Jan ; Fürtig, Boris ; Grün, J. Tassilo ; Hengesbach, Martin ; Hymon, Daniel ; Jonker, Hendrik R. A. ; Knezic, Bozana ; Korn, Sophie M. ; Landgraf, Tom ; Löhr, Frank ; Peter, Stephen A. ; Pyper, Dennis J. ; Qureshi, Nusrat S. ; Schlundt, Andreas ; Schnieders, Robbin ; Stirnal, Elke ; Sudakov, Alexey ; Vögele, Jennifer ; Weigand, Julia E. ; Wirmer-Bartoschek, Julia ; Witt, Kerstin ; Wöhnert, Jens ; Schwalbe, Harald ; Wacker, Anna (2024)
¹H, ¹³C and ¹⁵N assignment of stem-loop SL1 from the 5'-UTR of SARS-CoV-2.
In: Biomolecular NMR Assignments, 2021, 15 (2)
doi: 10.26083/tuprints-00023514
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Item Type: Article
Type of entry: Secondary publication
Title: ¹H, ¹³C and ¹⁵N assignment of stem-loop SL1 from the 5'-UTR of SARS-CoV-2
Language: English
Date: 30 September 2024
Place of Publication: Darmstadt
Year of primary publication: 2021
Place of primary publication: Dordrecht
Publisher: Springer Netherlands
Journal or Publication Title: Biomolecular NMR Assignments
Volume of the journal: 15
Issue Number: 2
DOI: 10.26083/tuprints-00023514
Corresponding Links:
Origin: Secondary publication DeepGreen
Abstract:

The stem-loop (SL1) is the 5'-terminal structural element within the single-stranded SARS-CoV-2 RNA genome. It is formed by nucleotides 7–33 and consists of two short helical segments interrupted by an asymmetric internal loop. This architecture is conserved among Betacoronaviruses. SL1 is present in genomic SARS-CoV-2 RNA as well as in all subgenomic mRNA species produced by the virus during replication, thus representing a ubiquitous cis-regulatory RNA with potential functions at all stages of the viral life cycle. We present here the ¹H, ¹³C and ¹⁵N chemical shift assignment of the 29 nucleotides-RNA construct 5_SL1, which denotes the native 27mer SL1 stabilized by an additional terminal G-C base-pair.

Uncontrolled Keywords: SARS-CoV-2, 5'-UTR, SL1, Solution NMR spectroscopy, COVID19-NMR
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-235142
Classification DDC: 500 Science and mathematics > 540 Chemistry
500 Science and mathematics > 570 Life sciences, biology
Divisions: 10 Department of Biology > RNA Biochemistry
Date Deposited: 30 Sep 2024 12:07
Last Modified: 05 Nov 2024 07:42
SWORD Depositor: Deep Green
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/23514
PPN: 523109121
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