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Label-free single-cell counting and characterization in the GHz-range

Gwozdz, Paul V. ; Harberts, Jann ; Zierold, Robert ; Blick, Robert H. (2023)
Label-free single-cell counting and characterization in the GHz-range.
In: Frequenz, 2022, 76 (11-12)
doi: 10.26083/tuprints-00023216
Article, Secondary publication, Publisher's Version

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Item Type: Article
Type of entry: Secondary publication
Title: Label-free single-cell counting and characterization in the GHz-range
Language: English
Date: 1 March 2023
Place of Publication: Darmstadt
Year of primary publication: 2022
Publisher: De Gruyter
Journal or Publication Title: Frequenz
Volume of the journal: 76
Issue Number: 11-12
DOI: 10.26083/tuprints-00023216
Corresponding Links:
Origin: Secondary publication

We demonstrate operation of a micropore based flow cytometer in the radio-frequency range. Apart from simply counting micron sized particles, such as cells, with close to nano-second resolution this counter offers the additional benefit of delivering insight into the intracellular environment. Such non-invasive screening of the cell’s interior based on analysing amplitude and phase of the signal is helpful in characterizing the biological activity of cells. In detail we are using heterodyne mixing to demodulate the temporal impedance changes, which are induced by cells translocating through a micropore embedded in a radio-frequency circuit. This allows us to measure every amplitude and phase modulation induced by a translocation event. Herein, we compare the Jurkat cells (human T lymphocytes) recordings with a control group of polystyrene beads. As the cells are measured on a single cell level, the variations on the measured amplitude and phase signals are used, herein, to sense morphological cell changes in real time.

Uncontrolled Keywords: radio frequency circuits, single cell counting, spectroscopic probing
Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-232167
Classification DDC: 500 Naturwissenschaften und Mathematik > 530 Physik
500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie
600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften und Maschinenbau
Divisions: 18 Department of Electrical Engineering and Information Technology > Institute for Microwave Engineering and Photonics (IMP)
Date Deposited: 01 Mar 2023 10:00
Last Modified: 27 Oct 2023 08:38
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/23216
PPN: 512716323
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