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Label-free single-cell counting and characterization in the GHz-range

Gwozdz, Paul V. ; Harberts, Jann ; Zierold, Robert ; Blick, Robert H. (2023):
Label-free single-cell counting and characterization in the GHz-range. (Publisher's Version)
In: Frequenz, 76 (11-12), pp. 719-728. De Gruyter, ISSN 0016-1136,
DOI: 10.26083/tuprints-00023216,
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Item Type: Article
Origin: Secondary publication
Status: Publisher's Version
Title: Label-free single-cell counting and characterization in the GHz-range
Language: English
Abstract:

We demonstrate operation of a micropore based flow cytometer in the radio-frequency range. Apart from simply counting micron sized particles, such as cells, with close to nano-second resolution this counter offers the additional benefit of delivering insight into the intracellular environment. Such non-invasive screening of the cell’s interior based on analysing amplitude and phase of the signal is helpful in characterizing the biological activity of cells. In detail we are using heterodyne mixing to demodulate the temporal impedance changes, which are induced by cells translocating through a micropore embedded in a radio-frequency circuit. This allows us to measure every amplitude and phase modulation induced by a translocation event. Herein, we compare the Jurkat cells (human T lymphocytes) recordings with a control group of polystyrene beads. As the cells are measured on a single cell level, the variations on the measured amplitude and phase signals are used, herein, to sense morphological cell changes in real time.

Journal or Publication Title: Frequenz
Volume of the journal: 76
Issue Number: 11-12
Place of Publication: Darmstadt
Publisher: De Gruyter
Uncontrolled Keywords: radio frequency circuits, single cell counting, spectroscopic probing
Classification DDC: 500 Naturwissenschaften und Mathematik > 530 Physik
500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie
600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften
Divisions: 18 Department of Electrical Engineering and Information Technology > Institute for Microwave Engineering and Photonics (IMP)
Date Deposited: 01 Mar 2023 10:00
Last Modified: 01 Mar 2023 10:00
DOI: 10.26083/tuprints-00023216
Corresponding Links:
URN: urn:nbn:de:tuda-tuprints-232167
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/23216
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