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Spread Layers of Lysozyme Microgel at Liquid Surface

Milyaeva, Olga Yu. ; Akentiev, Alexander V. ; Bykov, Alexey G. ; Lin, Shi-Yow ; Loglio, Giuseppe ; Miller, Reinhard ; Michailov, Alexander V. ; Rotanova, Ksenia Yu. ; Noskov, Boris A. (2022):
Spread Layers of Lysozyme Microgel at Liquid Surface. (Publisher's Version)
In: Polymers, 14 (19), MDPI, e-ISSN 2073-4360,
DOI: 10.26083/tuprints-00022486,

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Item Type: Article
Origin: Secondary publication DeepGreen
Status: Publisher's Version
Title: Spread Layers of Lysozyme Microgel at Liquid Surface
Language: English

The spread layers of lysozyme (LYS) microgel particles were studied by surface dilational rheology, infrared reflection–absorption spectra, Brewster angle microscopy, atomic force microscopy, and scanning electron microscopy. It is shown that the properties of LYS microgel layers differ significantly from those of ß-lactoglobulin (BLG) microgel layers. In the latter case, the spread protein layer is mainly a monolayer, and the interactions between particles lead to the increase in the dynamic surface elasticity by up to 140 mN/m. In contrast, the dynamic elasticity of the LYS microgel layer does not exceed the values for pure protein layers. The compression isotherms also do not exhibit specific features of the layer collapse that are characteristic for the layers of BLG aggregates. LYS aggregates form trough three-dimensional clusters directly during the spreading process, and protein spherulites do not spread further along the interface. As a result, the liquid surface contains large, almost empty regions and some patches of high local concentration of the microgel particles.

Journal or Publication Title: Polymers
Volume of the journal: 14
Issue Number: 19
Place of Publication: Darmstadt
Publisher: MDPI
Collation: 12 Seiten
Uncontrolled Keywords: β-lactoglobulin, lysozyme, microgel particles, spread layers, IRRAS, BAM, AFM, SEM, surface dilational viscoelasticity
Classification DDC: 500 Naturwissenschaften und Mathematik > 530 Physik
600 Technik, Medizin, angewandte Wissenschaften > 660 Technische Chemie
Divisions: 05 Department of Physics > Institute for Condensed Matter Physics
Date Deposited: 24 Oct 2022 13:18
Last Modified: 28 Oct 2022 06:19
DOI: 10.26083/tuprints-00022486
Corresponding Links:
URN: urn:nbn:de:tuda-tuprints-224869
Additional Information:

This article belongs to the Section Polymer Chemistry

SWORD Depositor: Deep Green
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/22486
PPN: 500845174
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