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Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays

Elter, Adrian Elias Curd ; Bock, Tina ; Spiehl, Dieter ; Russo, Giulio ; Hinz, Steffen C. ; Bitsch, Sebastian ; Baum, Eva ; Langhans, Markus ; Meckel, Tobias ; Dörsam, Edgar ; Kolmar, Harald ; Schwall, Gerhard (2022)
Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays.
In: Scientific Reports, 2022, 11
doi: 10.26083/tuprints-00021201
Article, Secondary publication, Publisher's Version

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Item Type: Article
Type of entry: Secondary publication
Title: Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays
Language: English
Date: 3 May 2022
Place of Publication: Darmstadt
Year of primary publication: 2022
Publisher: Springer Nature
Journal or Publication Title: Scientific Reports
Volume of the journal: 11
Collation: 11 Seiten
DOI: 10.26083/tuprints-00021201
Corresponding Links:
Origin: Secondary publication via sponsored Golden Open Access
Abstract:

Since the pandemic outbreak of Covid-19 in December 2019, several lateral flow assay (LFA) devices were developed to enable the constant monitoring of regional and global infection processes. Additionally, innumerable lateral flow test devices are frequently used for determination of different clinical parameters, food safety, and environmental factors. Since common LFAs rely on non-biodegradable nitrocellulose membranes, we focused on their replacement by cellulose-composed, biodegradable papers. We report the development of cellulose paper-based lateral flow immunoassays using a carbohydrate-binding module-fused to detection antibodies. Studies regarding the protein binding capacity and potential protein wash-off effects on cellulose paper demonstrated a 2.7-fold protein binding capacity of CBM-fused antibody fragments compared to the sole antibody fragment. Furthermore, this strategy improved the spatial retention of CBM-fused detection antibodies to the test area, which resulted in an enhanced sensitivity and improved overall LFA-performance compared to the naked detection antibody. CBM-assisted antibodies were validated by implementation into two model lateral flow test devices (pregnancy detection and the detection of SARS-CoV-2 specific antibodies). The CBM-assisted pregnancy LFA demonstrated sensitive detection of human gonadotropin (hCG) in synthetic urine and the CBM-assisted Covid-19 antibody LFA was able to detect SARS-CoV-2 specific antibodies present in serum. Our findings pave the way to the more frequent use of cellulose-based papers instead of nitrocellulose in LFA devices and thus potentially improve the sustainability in the field of POC diagnostics.

Status: Publisher's Version
URN: urn:nbn:de:tuda-tuprints-212014
Classification DDC: 500 Science and mathematics > 540 Chemistry
500 Science and mathematics > 570 Life sciences, biology
600 Technology, medicine, applied sciences > 600 Technology
Divisions: 07 Department of Chemistry > Clemens-Schöpf-Institut > Fachgebiet Biochemie
Date Deposited: 03 May 2022 11:22
Last Modified: 14 Nov 2023 19:04
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/21201
PPN: 494203633
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