Kukovetz, Kerri ; Hertel, Brigitte ; Schvarcz, Christopher R. ; Saponaro, Andrea ; Manthey, Mirja ; Burk, Ulrike ; Greiner, Timo ; Steward, Grieg F. ; Van Etten, James L. ; Moroni, Anna ; Thiel, Gerhard ; Rauh, Oliver (2021)
A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae.
In: Viruses, 2020, 12 (10)
doi: 10.26083/tuprints-00019251
Article, Secondary publication, Publisher's Version
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Item Type: | Article |
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Type of entry: | Secondary publication |
Title: | A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae |
Language: | English |
Date: | 2021 |
Year of primary publication: | 2020 |
Publisher: | MDPI |
Journal or Publication Title: | Viruses |
Volume of the journal: | 12 |
Issue Number: | 10 |
Collation: | 14 Seiten |
DOI: | 10.26083/tuprints-00019251 |
Corresponding Links: | |
Origin: | Secondary publication via sponsored Golden Open Access |
Abstract: | Potassium ion (K+) channels have been observed in diverse viruses that infect eukaryotic marine and freshwater algae. However, experimental evidence for functional K+ channels among these alga-infecting viruses has thus far been restricted to members of the family Phycodnaviridae, which are large, double-stranded DNA viruses within the phylum Nucleocytoviricota. Recent sequencing projects revealed that alga-infecting members of Mimiviridae, another family within this phylum, may also contain genes encoding K+ channels. Here we examine the structural features and the functional properties of putative K+ channels from four cultivated members of Mimiviridae. While all four proteins contain variations of the conserved selectivity filter sequence of K+ channels, structural prediction algorithms suggest that only two of them have the required number and position of two transmembrane domains that are present in all K+ channels. After in vitro translation and reconstitution of the four proteins in planar lipid bilayers, we confirmed that one of them, a 79 amino acid protein from the virus Tetraselmis virus 1 (TetV-1), forms a functional ion channel with a distinct selectivity for K+ over Na+ and a sensitivity to Ba2+. Thus, virus-encoded K+ channels are not limited to Phycodnaviridae but also occur in the members of Mimiviridae. The large sequence diversity among the viral K+ channels implies multiple events of lateral gene transfer. |
Status: | Publisher's Version |
URN: | urn:nbn:de:tuda-tuprints-192518 |
Classification DDC: | 500 Science and mathematics > 570 Life sciences, biology |
Divisions: | 10 Department of Biology > Plant Membrane Biophyscis (20.12.23 renamed in Biology of Algae and Protozoa) |
Date Deposited: | 03 Aug 2021 07:28 |
Last Modified: | 03 Aug 2021 07:28 |
URI: | https://tuprints.ulb.tu-darmstadt.de/id/eprint/19251 |
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