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Ionizing Radiation Induces Morphological Changes and Immunological Modulation of Jurkat Cells

Voos, Patrick and Fuck, Sebastian and Weipert, Fabian and Babel, Laura and Tandl, Dominique and Meckel, Tobias and Hehlgans, Stephanie and Fournier, Claudia and Moroni, Anna and Rödel, Franz and Thiel, Gerhard (2018):
Ionizing Radiation Induces Morphological Changes and Immunological Modulation of Jurkat Cells.
9, In: Frontiers in Immunology, ISSN 1664-3224,
DOI: 10.3389/fimmu.2018.00922,
Secondary publishing via sponsored Golden Open Access, [Article]

Available under CC-BY 4.0 International - Creative Commons, Attribution.

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Item Type: Article
Origin: Secondary publishing via sponsored Golden Open Access
Title: Ionizing Radiation Induces Morphological Changes and Immunological Modulation of Jurkat Cells
Language: English

Impairment or stimulation of the immune system by ionizing radiation (IR) impacts on immune surveillance of tumor cells and non-malignant cells and can either foster therapy response or side effects/toxicities of radiation therapy. For a better understanding of the mechanisms by which IR modulates T-cell activation and alters functional properties of these immune cells, we exposed human immortalized Jurkat cells and peripheral blood lymphocytes (PBL) to X-ray doses between 0.1 and 5 Gy. This resulted in cellular responses, which are typically observed also in naïve T-lymphocytes in response of T-cell receptor immune stimulation or mitogens. These responses include oscillations of cytosolic Ca2+, an upregulation of CD25 surface expression, interleukin-2 and interferon-γ synthesis, elevated expression of Ca2+ sensitive K+ channels and an increase in cell diameter. The latter was sensitive to inhibition by the immunosuppressant cyclosporine A, Ca2+ buffer BAPTA-AM, and the CDK1-inhibitor RO3306, indicating the involvement of Ca2+-dependent immune activation and radiation-induced cell cycle arrest. Furthermore, on a functional level, Jurkat and PBL cell adhesion to endothelial cells was increased upon radiation exposure and was highly dependent on an upregulation of integrin beta-1 expression and clustering. In conclusion, we here report that IR impacts on immune activation and functional properties of T-lymphocytes that may have implications in both toxic effects and treatment response to combined radiation and immune therapy in cancer patients.

Journal or Publication Title: Frontiers in Immunology
Volume: 9
Classification DDC: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie
Divisions: 10 Department of Biology
Date Deposited: 28 May 2018 12:11
Last Modified: 09 Jul 2020 02:07
DOI: 10.3389/fimmu.2018.00922
URN: urn:nbn:de:tuda-tuprints-74493
URI: https://tuprints.ulb.tu-darmstadt.de/id/eprint/7449
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